ArticleTissue engineering. Part A2009
Enhanced proliferation of human umbilical vein endothelial cells and differentiation of 3T3-L1 adipocytes in coculture.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
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- Danhong Injection Up-regulates miR-125b in Endothelial Exosomes and Attenuates Apoptosis in Post-Infarction Myocardium.Chinese journal of integrative medicine · 2023Article
- Successful fat-only whole breast reconstruction using cultured mature adipocytes and conditioned medium containing MCP-1.Scientific reports · 2023Article
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- Biomimetic on-chip assay reveals the anti-metastatic potential of a novel thienopyrimidine compound in triple-negative breast cancer cell lines.Frontiers in bioengineering and biotechnology · 2023Article
- Human umbilical vein endothelial cells-derived exosomes enhance cardiac function after acute myocardial infarction by activating the PI3K/AKT signaling pathway.Bioengineered · 2022Article
- Perspectives on scaling production of adipose tissue for food applications.Biomaterials · 2022Article
- Endothelial cell crosstalk improves browning but hinders white adipocyte maturation in 3D engineered adipose tissue.Integrative biology : quantitative biosciences from nano to macro · 2020Article
- Cell alignment and accumulation using acoustic nozzle for bioprinting.Scientific reports · 2019Article
- Silicon-Enhanced Adipogenesis and Angiogenesis for Vascularized Adipose Tissue Engineering.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Angiogenic, lymphangiogenic and adipogenic effects of HIV-1 matrix protein p17.Pathogens and disease · 2015Article
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- The secretion pattern of perivascular fat cells is different from that of subcutaneous and visceral fat cells.Diabetologia · 2012Article
- Effect of soluble Jagged1-mediated inhibition of Notch signaling on proliferation and differentiation of an adipocyte progenitor cell model.Adipocyte · 2012Article
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- Lipolytic function of adipocyte/endothelial cocultures.Tissue engineering. Part A · 2011Article
- Immunohistochemical and transcriptional expression of matrix metalloproteinases in full-term human umbilical cord and human umbilical vein endothelial cells.Journal of molecular histology · 2010Article
- Effects of hyperinsulinemia on lipolytic function of three-dimensional adipocyte/endothelial co-cultures.Tissue engineering. Part C, Methods · 2010Article
- Adipose tissue engineering for soft tissue regeneration.Tissue engineering. Part B, Reviews · 2010Review
- Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
The interactions between adipocytes and endothelial cells in adipose tissue development are poorly understood. In this study, we characterized the growth and differentiation of 3T3-L1 preadipocytes and human umbilical vein endothelial cells (HUVECs) in planar and collagen gel cocultures. In planar coculture, preadipocyte proliferation was up to three times as great as in the control culture with only preadipocytes, where the increase was proportional to the HUVEC fraction in the seeding mixture. In the collagen gel coculture, triglyceride (TG) content (per adipocyte) was up to 3.4 times as much as in the control with only adipocytes. This effect depended on the total density and composition of the seeding mixture, with the largest increase observed at the highest density (2 x 10(6) cells/mL collagen) and preadipocyte:HUVEC ratio (90:10) tested in this study. Immunostaining showed that the collagen gel coculture also supported the elongation of endothelial cells. Blockade of vascular endothelial growth factor receptor 2 (VEGFR2) abolished the adipogenesis- and neovascularization-related effects of the coculture. Taken together, our results indicate that endothelial cell-mediated enhancement of adipocyte differentiation requires the activation of VEGFR2.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.