ArticleProtoplasma2008
Plasmodesmata transport of GFP alone or fused to potato virus X TGBp1 is diffusion driven.
Article in Protoplasma, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
10 citing papers in PubMed, 40 citations in OpenAlex.
- Distributing Plant Developmental Regulatory Proteins via Plasmodesmata.Plants (Basel, Switzerland) · 2024Review
- Intercellular trafficking via plasmodesmata: molecular layers of complexity.Cellular and molecular life sciences : CMLS · 2021Review
- Review
- From plasmodesma geometry to effective symplasmic permeability through biophysical modelling.eLife · 2019Article
- Exposure to heavy metal stress triggers changes in plasmodesmatal permeability via deposition and breakdown of callose.Journal of experimental botany · 2018Article
- Potato virus X movement in Nicotiana benthamiana: new details revealed by chimeric coat protein variants.Molecular plant pathology · 2012Article
- Plasmodesmata viewed as specialised membrane adhesion sites.Protoplasma · 2011Review
- An Arabidopsis GPI-anchor plasmodesmal neck protein with callose binding activity and potential to regulate cell-to-cell trafficking.The Plant cell · 2009Article
- Diffusion of anionic and neutral GFP derivatives through plasmodesmata in epidermal cells of Nicotiana benthamiana.Protoplasma · 2008Article
- Plasmodesmata transport of GFP and GFP fusions requires little energy and transitions during leaf expansion.Plant signaling & behavior · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plasmodesmata (Pd) provide a pathway for exchanging various macromolecules between neighboring plant cells. Researchers routinely characterize the mobility of the green-fluorescent protein (GFP) and GFP fusions through Pd by calculating the proportion of sites in bombarded leaves which show fluorescence in multiple cell clusters (% movement). Here, the Arrhenius equation was used to describe the temperature dependence of GFP and GFP-TGBpl (potato virus X triple gene block protein1) movement, using % movement values, and to calculate the activation energy for protein transport. The resulting low activation energy indicates GFP and GFP-TGBp1 movement are diffusion driven. Furthermore, GFP movement is inversely proportional to the leaf surface area of expanding leaves. The increase in leaf area results mainly from cell expansion during the sink-source transition. The increasing cell size results in lower Pd density, which decreases the probability that a GFP attains an open Pd by diffusion. The decline in GFP movement as leaf area expands indicates that, in addition to GFP diffusion through Pd, attaining an open Pd by undirected diffusion might be limiting for Pd transport. In summary, this report provides a new quantitative method for studying Pd conductivity.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.