ArticleJournal of cell communication and signaling2008
Enzymes used in molecular biology: a useful guide.
Article in Journal of cell communication and signaling, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 104 citations in OpenAlex.
- Establishing Practical and Cost-Effective Methodologies for Preservation and Extraction of Environmental RNA (eRNA) From Aquatic Samples.Molecular ecology resources · 2026Article
- Recent advances in designing high-performance and enzymatically degradable plastics.Chemical science · 2026Review
- Polymerase Chain Reaction: A Toolbox for Molecular Discovery.Molecular biotechnology · 2026Review
- Viral Dark Matter: Illuminating Protein Function, Ecology, and Biotechnological Promises.Biochemistry · 2025Review
- RoCK and ROI: single-cell transcriptomics with multiplexed enrichment of selected transcripts and region-specific sequencing.Nature communications · 2025Article
- Cellulose and cellulose-based materials for enzyme immobilization: a review.Biophysical reviews · 2025Review
- Viral Dark Matter: Illuminating Protein Function, Ecology, and Biotechnological Promises.ArXiv · 2025Article
- Cold-active microbial enzymes and their biotechnological applications.Microbial biotechnology · 2024Review
- Electrochemical DNA Cleavage Sensing for EcoRV Activity and Inhibition with an ERGO Electrode.Biosensors · 2024Article
- Isolation of High-Molecular-Weight DNA for Long-Read Sequencing Using a High-Salt Gel Electroelution Trap.Analytical chemistry · 2023Article
- Review
- The development of recycling methods for bio-based materials - A challenge in the implementation of a circular economy: A review.Waste management & research : the journal of the International Solid Wastes and Public Cleansing Association, ISWA · 2023Review
- Article
- Thermal stabilization of diverse biologics using reversible hydrogels.Science advances · 2022Article
- Modelling of Environmental Ageing of Polymers and Polymer Composites-Modular and Multiscale Methods.Polymers · 2022Review
- Loop-Mediated Isothermal Amplification: From Theory to Practice.Russian journal of bioorganic chemistry · 2022Article
- Approaching Sites of Action of Temozolomide for Pharmacological and Clinical Studies in Glioblastoma.Biomedicines · 2021Article
- Magnesium magnetic isotope effects in microbiology.Archives of microbiology · 2021Review
- Quick and Easy Assembly of a One-Step qRT-PCR Kit for COVID-19 Diagnostics Using In-House Enzymes.ACS omega · 2021Article
- An Update on Molecular Tools for Genetic Engineering of Actinomycetes-The Source of Important Antibiotics and Other Valuable Compounds.Antibiotics (Basel, Switzerland) · 2020Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing the readers with some cues for understanding the function and specificities of the different sources of polymerases, ligases, nucleases, phosphatases, methylases, and topoisomerases used for molecular cloning. We provide a description of the most commonly used enzymes of each group, and explain their properties and mechanism of action. By pointing out key requirements for each enzymatic activity and clarifying their limitations, we aim at guiding the reader in selecting appropriate enzymatic source and optimal experimental conditions for molecular cloning experiments.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.