ArticleCell biochemistry and biophysics2008
Endothelial injury induces vascular smooth muscle cell proliferation in highly localized regions of a direct contact co-culture system.
Article in Cell biochemistry and biophysics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 24 citations in OpenAlex.
- Manufacturing, Cell Regulation, and Coculture Strategies for Vascularization and Neural Innervation of Skeletal Muscle Tissue.Advanced healthcare materials · 2026Review
- Heparinized Elastomeric Nanofibrillar Grafts: Mechanically Tunable, Cell-Supportive, and Thromboresistant Vascular Substitutes.ACS applied bio materials · 2026Article
- Heparinized Elastomeric Nanofibrillar Grafts: A Novel Approach for Mechanically Tunable, Cell-Supportive, and Thromboresistant Vascular Substitutes.bioRxiv : the preprint server for biology · 2026Article
- Metabolic score for visceral fat profiles and incident cardiovascular disease in adults with CKM syndrome stages 0-3: a CHARLS longitudinal study.BMC cardiovascular disorders · 2026Article
- Endothelial-smooth muscle microgauges for modeling pulmonary arterial vasoregulation.Lab on a chip · 2025Article
- Endothelial-Smooth Muscle Cell Interactions in a Shear-Exposed Intimal Hyperplasia on-a-Dish Model to Evaluate Therapeutic Strategies.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
- Design considerations for engineering 3D models to study vascular pathologies in vitro.Acta biomaterialia · 2021Review
- The pro-atherogenic response to disturbed blood flow is increased by a western diet, but not by old age.Scientific reports · 2019Article
- Endothelial-Vascular Smooth Muscle Cells Interactions in Atherosclerosis.Frontiers in cardiovascular medicine · 2018Review
- A Cell Culture Model of Resistance Arteries.Journal of visualized experiments : JoVE · 2017Article
- Amniotic fluid-derived stem cells demonstrated cardiogenic potential in indirect co-culture with human cardiac cells.Annals of biomedical engineering · 2014Article
- Endothelial control of vasodilation: integration of myoendothelial microdomain signalling and modulation by epoxyeicosatrienoic acids.Pflugers Archiv : European journal of physiology · 2014Review
- Formation of functional gap junctions in amniotic fluid-derived stem cells induced by transmembrane co-culture with neonatal rat cardiomyocytes.Journal of cellular and molecular medicine · 2013Article
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2 authors at 2 institutions in 1 country.
Funding
Abstract
Though previous studies have indicated a relationship between the proliferation of endothelial cells and vascular smooth muscle cells (VSMCs) in co-culture, the results have been contradictory and the signaling mechanism poorly understood. In this transmembrane co-culture study, VSMCs and endothelial cells were grown to confluence on opposite sides of a microporous membrane to mimic the intima/media border of vessels. The endothelial layer was injured, and then cultured for 3 days, resulting in partial re-endothelialization. VSMC proliferation across from the injured/partially recovered endothelial region was significantly higher than across from the de-endothelialized region (a sevenfold increase) and the uninjured region (a threefold increase). ELISA indicated that PDGF, which was undetectable in uninjured co-culture and homotypic controls, increased after injury and the addition of a piperazinyl-quinazoline carboxamide PDGF receptor inhibitor blocked VSMC proliferation across from the injured/partially recovered region. We conclude that co-culture signaling initiated by endothelial cell injury locally stimulates VSMC proliferation and that this signaling could be mediated by PDGF-BB.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.