ArticleProceedings of the National Academy of Sciences of the United States of America2008
Regulation of TATA-binding protein dynamics in living yeast cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed.
- Genome-scale chromatin binding dynamics of RNA Polymerase II general transcription machinery components.The EMBO journal · 2024Article
- Impact ofInternational journal of molecular sciences · 2022Review
- Bound2Learn: a machine learning approach for classification of DNA-bound proteins from single-molecule tracking experiments.Nucleic acids research · 2021Article
- Molecular determinants underlying functional innovations of TBP and their impact on transcription initiation.Nature communications · 2020Article
- Kin28 depletion increases association of TFIID subunits Taf1 and Taf4 with promoters in Saccharomyces cerevisiae.Nucleic acids research · 2020Article
- Conformational changes and catalytic inefficiency associated with Mot1-mediated TBP-DNA dissociation.Nucleic acids research · 2019Article
- Single-molecule imaging correlates decreasing nuclear volume with increasing TF-chromatin associations during zebrafish development.Nature communications · 2018Article
- Splicing heterogeneity: separating signal from noise.Genome biology · 2018Article
- Live-cell analysis of endogenous GFP-RPB1 uncovers rapid turnover of initiating and promoter-paused RNA Polymerase II.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Viral targeting of TFIIB impairs de novo polymerase II recruitment and affects antiviral immunity.PLoS pathogens · 2018Article
- Coactivators and general transcription factors have two distinct dynamic populations dependent on transcription.The EMBO journal · 2017Article
- RNA synthesis is associated with multiple TBP-chromatin binding events.Scientific reports · 2017Article
- Molecular Mechanism of Mot1, a TATA-binding Protein (TBP)-DNA Dissociating Enzyme.The Journal of biological chemistry · 2016Article
- Affinity and competition for TBP are molecular determinants of gene expression noise.Nature communications · 2016Article
- Regulation of anti-sense transcription by Mot1p and NC2 via removal of TATA-binding protein (TBP) from the 3'-end of genes.Nucleic acids research · 2015Article
- Suppression of intragenic transcription requires the MOT1 and NC2 regulators of TATA-binding protein.Nucleic acids research · 2014Article
- Independent RNA polymerase II preinitiation complex dynamics and nucleosome turnover at promoter sites in vivo.Genome research · 2014Article
- Mot1 redistributes TBP from TATA-containing to TATA-less promoters.Molecular and cellular biology · 2013Article
- Measuring chromatin interaction dynamics on the second time scale at single-copy genes.Science (New York, N.Y.) · 2013Article
- Eukaryotic transcriptional dynamics: from single molecules to cell populations.Nature reviews. Genetics · 2013Review
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Although pathways for assembly of RNA polymerase (Pol) II transcription preinitiation complexes (PICs) have been well established in vitro, relatively little is known about the dynamic behavior of Pol II general transcription factors in vivo. In vitro, a subset of Pol II factors facilitates reinitiation by remaining very stably bound to the promoter. This behavior contrasts markedly with the highly dynamic behavior of RNA Pol I transcription complexes in vivo, which undergo cycles of disassembly/reassembly at the promoter for each round of transcription. To determine whether the dynamic behavior of the Pol II machinery in vivo is fundamentally different from that of Pol I and whether the static behavior of Pol II factors in vitro fully recapitulates their behavior in vivo, we used fluorescence recovery after photobleaching (FRAP). Surprisingly, we found that all or nearly all of the TATA-binding protein (TBP) population is highly mobile in vivo, displaying FRAP recovery rates of <15 s. These high rates require the activity of the TBP-associated factor Mot1, suggesting that TBP/chromatin interactions are destabilized by active cellular processes. Furthermore, the distinguishable FRAP behavior of TBP and TBP-associated factor 1 indicates that there are populations of these molecules that are independent of one another. The distinct FRAP behavior of most Pol II factors that we tested suggests that transcription complexes assemble via stochastic multistep pathways. Our data indicate that active Pol II PICs can be much more dynamic than previously considered.
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