ArticleProceedings of the National Academy of Sciences of the United States of America2008
Muscle and nonmuscle myosins probed by a spin label at equivalent sites in the force-generating domain.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Structural and dynamic origins of ESR lineshapes in spin-labeled GB1 domain: the insights from spin dynamics simulations based on long MD trajectories.Scientific reports · 2020Article
- A Cardiomyopathy Mutation in the Myosin Essential Light Chain Alters Actomyosin Structure.Biophysical journal · 2017Article
- Macromolecular Crowding Modulates Actomyosin Kinetics.Biophysical journal · 2016Article
- High-resolution helix orientation in actin-bound myosin determined with a bifunctional spin label.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Electron paramagnetic resonance resolves effects of oxidative stress on muscle proteins.Exercise and sport sciences reviews · 2014Review
- Metal cation controls myosin and actomyosin kinetics.Protein science : a publication of the Protein Society · 2013Article
- Conformationally trapping the actin-binding cleft of myosin with a bifunctional spin label.The Journal of biological chemistry · 2013Article
- The mechanism of the converter domain rotation in the recovery stroke of myosin motor protein.Proteins · 2012Article
- Mn(2+)-nucleotide coordination at the myosin active site as detected by pulsed electron paramagnetic resonance.The journal of physical chemistry. B · 2012Article
- Allosteric communication in Dictyostelium myosin II.Journal of muscle research and cell motility · 2012Article
- Article
- Three distinct actin-attached structural states of myosin in muscle fibers.Biophysical journal · 2012Article
- Early stages of the recovery stroke in myosin II studied by molecular dynamics simulations.Protein science : a publication of the Protein Society · 2011Article
- Structural and functional impact of site-directed methionine oxidation in myosin.Biochemistry · 2011Article
- Structural kinetics of myosin by transient time-resolved FRET.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Article
- Phosphorylation-induced structural changes in smooth muscle myosin regulatory light chain.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Protein structural dynamics revealed by site-directed spin labeling and multifrequency EPR.Biophysical reviews · 2010Article
- Structural dynamics of the myosin relay helix by time-resolved EPR and FRET.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Site-directed spectroscopic probes of actomyosin structural dynamics.Annual review of biophysics · 2009Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
We have engineered a mutant of Dictyostelium discoideum (Dicty) myosin II that contains the same fast-reacting "SH1" thiol as in muscle myosin, spin-labeled it, and performed electron paramagnetic resonance (EPR) to compare the structure of the force-generating region of the two myosins. Dicty myosin serves as a model system for muscle myosin because of greater ease of mutagenesis, expression, and crystallization. The catalytic domains of these myosins have nearly identical crystal structures in the apo state, but there are significant differences in ATPase kinetics, and there are no crystal structures of skeletal muscle myosin with bound nucleotides, so another structural technique is needed. Previous EPR studies, with a spin label attached to SH1 in muscle myosin, have resolved the key structural states of this region. Therefore, we have performed identical experiments on both myosins spin-labeled at equivalent sites. Spectra were identical for the two myosins in the apo and ADP-bound states. With bound ADP and phosphate analogs, (i) both proteins exhibit two resolved structural states (prepowerstroke, postpowerstroke) in a single biochemical state (defined by the bound nucleotide), and (ii) these structural states are essentially identical in the two myosins but (iii) are occupied to different extents as a function of the biochemical state. We conclude that (i) myosin structural and biochemical states do not have a one-to-one correspondence, and (ii) Dicty myosin can serve as a good analog for structural studies of muscle myosin only if differences in the coupling between biochemical and structural states are taken into account.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.