ArticleInfection and immunity2008
Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 77 papers.
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Who cites it
77 citing papers in PubMed, 130 citations in OpenAlex.
- Environmental regulation of virulence in Vibrio cholerae: integrating multi-omics, predictive models, and comparative insights across emerging Vibrio species.Gut pathogens · 2026Review
- Tangled Up in Fibers: How a Multidomain Lytic Polysaccharide Monooxygenase Binds Its Chitin Substrate.ACS applied materials & interfaces · 2026Article
- Human antibody targetingmBio · 2025Article
- Clostridium perfringens chitinases, key enzymes during early stages of necrotic enteritis in broiler chickens.PLoS pathogens · 2024Article
- Elucidation of cellular signaling mechanism involved inInfectious medicine · 2024Article
- Calcium-binding site in AA10 LPMO fromQRB discovery · 2024Article
- Genetic and Phenotypic Virulence Potential of Non-O1/Non-O139Microorganisms · 2023Article
- Perdeuterated GbpA Enables Neutron Scattering Experiments of a Lytic Polysaccharide Monooxygenase.ACS omega · 2023Article
- A mucin-regulated adhesin determines the spatial organization and inflammatory character of a bacterial symbiont in the vertebrate gut.Cell host & microbe · 2023Article
- Priestia flexa KS1: A new bacterial strain isolated from human faeces implicated in mucin degradation.International microbiology : the official journal of the Spanish Society for Microbiology · 2023Article
- Bacterial Chitinases and Their Role in Human Infection.Infection and immunity · 2023Review
- Vibrio cholerae biofilms use modular adhesins with glycan-targeting and nonspecific surface binding domains for colonization.Nature communications · 2023Article
- Review
- Stress Responses in Pathogenic Vibrios and Their Role in Host and Environmental Survival.Advances in experimental medicine and biology · 2023Article
- Chitinolytic enzymes contribute to the pathogenicity of Aliivibrio salmonicida LFI1238 in the invasive phase of cold-water vibriosis.BMC microbiology · 2022Article
- The crystal structure of CbpD clarifies substrate-specificity motifs in chitin-active lytic polysaccharide monooxygenases.Acta crystallographica. Section D, Structural biology · 2022Article
- Vibrio cholerae senses human enteric α-defensin 5 through a CarSR two-component system to promote bacterial pathogenicity.Communications biology · 2022Article
- Salmonella enterica serovar Typhimurium chitinases modulate the intestinal glycome and promote small intestinal invasion.PLoS pathogens · 2022Article
- Starvation causes changes in the intestinal transcriptome and microbiome that are reversed upon refeeding.BMC genomics · 2022Article
- An Increase of Seawater Temperature Upregulates the Expression ofFrontiers in microbiology · 2022Article
17 more citing papers are in PubMed but not listed here.
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The chitin-binding protein GbpA of Vibrio cholerae has been recently described as a common adherence factor for chitin and intestinal surface. Using an isogenic in-frame gbpA deletion mutant, we first show that V. cholerae O1 El Tor interacts with mouse intestinal mucus quickly, using GbpA in a specific manner. The gbpA mutant strain showed a significant decrease in intestinal adherence, leading to less colonization and fluid accumulation in a mouse in vivo model. Purified recombinant GbpA (rGbpA) specifically bound to N-acetyl-D-glucosamine residues of intestinal mucin in a dose-dependent, saturable manner with a dissociation constant of 11.2 microM. Histopathology results from infected mouse intestine indicated that GbpA binding resulted in a time-dependent increase in mucus secretion. We found that rGbpA increased the production of intestinal secretory mucins (MUC2, MUC3, and MUC5AC) in HT-29 cells through upregulation of corresponding genes. The upregulation of MUC2 and MUC5AC genes was dependent on NF-kappaB nuclear translocation. Interestingly, mucin could also increase GbpA expression in V. cholerae in a dose-dependent manner. Thus, we propose that there is a coordinated interaction between GbpA and mucin to upregulate each other in a cooperative manner, leading to increased levels of expression of both of these interactive factors and ultimately allowing successful intestinal colonization and pathogenesis by V. cholerae.
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