ArticleThe Journal of biological chemistry2008
Drosophila MFAP1 is required for pre-mRNA processing and G2/M progression.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Article
- Sustainable integrative cell biology: CENP-C is guilty by association.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2025Article
- Defining Splicing Factor Requirements for Androgen Receptor Variant Synthesis in Advanced Prostate Cancer.Molecular cancer research : MCR · 2024Article
- Xrp1 governs the stress response program to spliceosome dysfunction.Nucleic acids research · 2024Article
- Spliceosome assembly and regulation: insights from analysis of highly reduced spliceosomes.RNA (New York, N.Y.) · 2023Review
- Article
- Review
- Somatic CG6015 mediates cyst stem cell maintenance and germline stem cell differentiation via EGFR signaling in Drosophila testes.Cell death discovery · 2021Article
- Tyramide signal amplification mass spectrometry (TSA-MS) ratio identifies nuclear speckle proteins.The Journal of cell biology · 2020Article
- Depletion of the MFAP1/SPP381 Splicing Factor Causes R-Loop-Independent Genome Instability.Cell reports · 2019Article
- Prp8 regulates oncogene-induced hyperplastic growth inDevelopment (Cambridge, England) · 2018Article
- Therapy-induced stress response is associated with downregulation of pre-mRNA splicing in cancer cells.Genome medicine · 2018Article
- Human THO-Sin3A interaction reveals new mechanisms to prevent R-loops that cause genome instability.The EMBO journal · 2017Article
- Quantitative Temporal Viromics of an Inducible HIV-1 Model Yields Insight to Global Host Targets and Phospho-Dynamics Associated with Protein Vpr.Molecular & cellular proteomics : MCP · 2017Article
- Human MFAP1 is a cryptic ortholog of the Saccharomyces cerevisiae Spp381 splicing factor.BMC evolutionary biology · 2017Article
- Article
- Mitotic control by mRNA splicing regulators ensures primary cilia formation.Animal cells and systems · 2017Article
- Multiple protein-protein interactions converging on the Prp38 protein during activation of the human spliceosome.RNA (New York, N.Y.) · 2016Article
- The microfibril-associated glycoproteins (MAGPs) and the microfibrillar niche.Matrix biology : journal of the International Society for Matrix Biology · 2015Review
- Graded requirement for the spliceosome in cell cycle progression.Cell cycle (Georgetown, Tex.) · 2015Article
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2 authors.
Funding
Abstract
The mammalian spliceosome has mainly been studied using proteomics. The isolation and comparison of different splicing intermediates has revealed the dynamic association of more than 200 splicing factors with the spliceosome, relatively few of which have been studied in detail. Here, we report the characterization of the Drosophila homologue of microfibril-associated protein 1 (dMFAP1), a previously uncharacterized protein found in some human spliceosomal fractions ( Jurica, M. S., and Moore, M. J. (2003) Mol. Cell 12, 5-14 ). We show that dMFAP1 binds directly to the Drosophila homologue of Prp38p (dPrp38), a tri-small nuclear ribonucleoprotein component ( Xie, J., Beickman, K., Otte, E., and Rymond, B. C. (1998) EMBO J. 17, 2938-2946 ), and is required for pre-mRNA processing. dMFAP1, like dPrp38, is essential for viability, and our in vivo data show that cells with reduced levels of dMFAP1 or dPrp38 proliferate more slowly than normal cells and undergo apoptosis. Consistent with this, double-stranded RNA-mediated depletion of dPrp38 or dMFAP1 causes cells to arrest in G(2)/M, and this is paralleled by a reduction in mRNA levels of the mitotic phosphatase string/cdc25. Interestingly double-stranded RNA-mediated depletion of a wide range of core splicing factors elicits a similar phenotype, suggesting that the observed G(2)/M arrest might be a general consequence of interfering with spliceosome function.
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