ArticleMolecular pharmaceutics
Characterization and real-time imaging of gene expression of adenovirus embedded silk-elastinlike protein polymer hydrogels.
Article in Molecular pharmaceutics. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 30 citations in OpenAlex.
- Bioengineered elastin- and silk-biomaterials for drug and gene delivery.Advanced drug delivery reviews · 2020Pooled it
- Translational Development of a Silk-Elastinlike Protein Polymer Embolic for Transcatheter Arterial Embolization.Macromolecular bioscience · 2022Article
- In vivo evaluation of matrix metalloproteinase responsive silk-elastinlike protein polymers for cancer gene therapy.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Imaging strategies for tissue engineering applications.Tissue engineering. Part B, Reviews · 2015Review
- Effect of shear on physicochemical properties of matrix metalloproteinase responsive silk-elastinlike hydrogels.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Silk-based biomaterials for sustained drug delivery.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Controlled release from recombinant polymers.Journal of controlled release : official journal of the Controlled Release Society · 2014Review
- Designing protein-based biomaterials for medical applications.Acta biomaterialia · 2014Review
- High level expression and facile purification of recombinant silk-elastin-like polymers in auto induction shake flask cultures.AMB Express · 2013Article
- Smart self-assembled hybrid hydrogel biomaterials.Angewandte Chemie (International ed. in English) · 2012Review
- Comparison of silk-elastinlike protein polymer hydrogel and poloxamer in matrix-mediated gene delivery.International journal of pharmaceutics · 2012Article
- Engineering biomaterial systems to enhance viral vector gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Silk-elastinlike recombinant polymers for gene therapy of head and neck cancer: from molecular definition to controlled gene expression.Journal of controlled release : official journal of the Controlled Release Society · 2009Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Transient expression levels, vector dissemination and toxicities associated with adenoviral vectors have prompted the usage of matrices for localized and controlled gene delivery. Two recombinant silk-elastinlike protein polymer analogues, SELP-47K and SELP-415K, consisting of different lengths and ratios of silk and elastin units, were previously shown to be injectable hydrogels capable of matrix-mediated controlled adenoviral gene delivery. Reported here is a study of spatiotemporal control over adenoviral gene expression with these SELP analogues in a human tumor xenograft model of head and neck cancer using whole animal imaging. Real-time images of viral expression levels indicate that polymer concentration and polymer structure are predominant factors that affect viral release and, thus, viral transfection. Decrease in polymer concentration and increase in polymer elastin content results in greater release, probably due to changes in the network structure of the hydrogel. To better understand this relationship, macro- and microstructural properties of the hydrogels were analyzed using dynamic mechanical analysis (DMA) and transmission electron microscopy (TEM). The results confirm that the concentration and the elastin content of the protein polymer affect the pore size of the hydrogel by changing the physical constraints of the SELP fibril network and the degree of hydration of the SELP fibrils. The potential to modulate viral release using SELP hydrogel delivery vehicles that can be injected intratumorally by minimally invasive techniques holds significant promise for the delivery of therapeutic viruses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.