ArticleGlycoconjugate journal2009
Knockdown of a galectin-1-like protein in zebrafish (Danio rerio) causes defects in skeletal muscle development.
Article in Glycoconjugate journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed.
- A Zebrafish Galectin-1 Isoform Is Expressed in Skin and Gills and Binds to Bacteria, Bacterial Adhesin Receptors, and Epidermal Mucus Glycans.International journal of molecular sciences · 2026Article
- Molecular Actors of Inflammation and Their Signaling Pathways: Mechanistic Insights from Zebrafish.Biology · 2023Review
- Structure-based identification of galectin-1 selective modulators in dietary food polyphenols: a pharmacoinformatics approach.Molecular diversity · 2022Article
- Manipulating Galectin Expression in Zebrafish (Danio rerio).Methods in molecular biology (Clifton, N.J.) · 2022Review
- Transcriptomic Response toFrontiers in genetics · 2021Article
- Integrated Glycoproteomics Identifies a Role of N-Glycosylation and Galectin-1 on Myogenesis and Muscle Development.Molecular & cellular proteomics : MCP · 2021Article
- Pregnancy Galectinology: Insights Into a Complex Network of Glycan Binding Proteins.Frontiers in immunology · 2019Review
- The zebrafish galectins Drgal1-L2 and Drgal3-L1 bind in vitro to the infectious hematopoietic necrosis virus (IHNV) glycoprotein and reduce viral adhesion to fish epithelial cells.Developmental and comparative immunology · 2016Article
- Article
- Manipulating galectin expression in zebrafish (Danio rerio).Methods in molecular biology (Clifton, N.J.) · 2015Article
- Evolving mechanistic insights into galectin functions.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Galectin-1 in early acute myocardial infarction.PloS one · 2014Article
- "Casting" light on the role of glycosylation during embryonic development: insights from zebrafish.Glycoconjugate journal · 2013Review
- Structural and functional diversity of the lectin repertoire in teleost fish: relevance to innate and adaptive immunity.Developmental and comparative immunology · 2011Review
- Diversified carbohydrate-binding lectins from marine resources.Journal of amino acids · 2011Article
- Crystallization and preliminary X-ray crystallographic analysis of zebrafish prototype galectin Drgal1-L2.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- Comparative analysis of crystallins and lipids from the lens of Antarctic toothfish and cow.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2010Article
- The zebrafish galectin Drgal1-l2 is expressed by proliferating Müller glia and photoreceptor progenitors and regulates the regeneration of rod photoreceptors.Investigative ophthalmology & visual science · 2010Article
- Differential expression of immunomodulatory galectin-1 in peripheral leukocytes and adult tissues and its cytosolic organization in striated muscle.Glycobiology · 2010Article
- Caenorhabditis elegans N-glycan core beta-galactoside confers sensitivity towards nematotoxic fungal galectin CGL2.PLoS pathogens · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
We previously identified and characterized four galectin-1-like proteins in zebrafish, Drgal1-L1, Drgal1-L2, Drgal1-L3, and one splice variant of Drgal1-L2, of distinct ontogenic expression. Drgal1-L1 is maternal; Drgal1-L2 is zygotic and strongly expressed in the notochord, while Drgal1-L3 is both maternal and zygotic. Knockdown experiments in zebrafish embryos using a morpholino-modified antisense oligo targeted to the 5'-UTR sequence of Drgal1-L2 resulted in a phenotype with a bent tail and disorganized muscle fibers. This effect was dose-dependent as follows: 62-66% at 17 ng, 29-35% at 5.7 ng, 21-28% at 1.9 ng, and 14-17% at 0.6 ng. However, no (or a negligible number of) Drgal1-L1 knockdown embryos showed similar morphological defects, indicating that the observed effects are sequence-specific, and not due to the toxicity of the morpholino-modified oligos. Further, ectopic expression of native Drgal1-L2 specifically rescued the phenotype, as co-injection of the full-length sense Drgal1-L2 mRNA with Drgal1-L2-MO yielded 60-62% normal embryos. As the notochord serves as the primary source of signaling molecules required for proper patterning of adjacent tissues, such as neural tube, somites, and heart, these results suggest that galectins produced by the notochord play a key role in somitic cell differentiation and development.
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