ArticleThe New phytologist2008
Ectopic expression of Arabidopsis thaliana plasma membrane intrinsic protein 2 aquaporins in lily pollen increases the plasma membrane water permeability of grain but not of tube protoplasts.
Article in The New phytologist, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 40 citations in OpenAlex.
- Transcriptome and Small RNA Sequencing Reveal the Mechanisms Regulating Harvest Index inFrontiers in plant science · 2022Article
- LRX Proteins Play a Crucial Role in Pollen Grain and Pollen Tube Cell Wall Development.Plant physiology · 2018Article
- Signaling with Ions: The Keystone for Apical Cell Growth and Morphogenesis in Pollen Tubes.Plant physiology · 2017Review
- Pollen-Specific Aquaporins NIP4;1 and NIP4;2 Are Required for Pollen Development and Pollination in Arabidopsis thaliana.The Plant cell · 2016Article
- Genome-Wide Analysis of the Aquaporin Gene Family in Chickpea (Frontiers in plant science · 2016Article
- Pollen Aquaporins: The Solute Factor.Frontiers in plant science · 2016Review
- Lost in traffic? The K(+) channel of lily pollen, LilKT1, is detected at the endomembranes inside yeast cells, tobacco leaves, and lily pollen.Frontiers in plant science · 2015Article
- Mercury-sensitive water channels as possible sensors of water potentials in pollen.Journal of experimental botany · 2013Article
- Sucrose-induced receptor kinase SIRK1 regulates a plasma membrane aquaporin in Arabidopsis.Molecular & cellular proteomics : MCP · 2013Article
- Control of cell wall extensibility during pollen tube growth.Molecular plant · 2013Review
- Water status and associated processes mark critical stages in pollen development and functioning.Annals of botany · 2012Review
- An osmotic model of the growing pollen tube.PloS one · 2012Article
- Single-molecule analysis of PIP2;1 dynamics and partitioning reveals multiple modes of Arabidopsis plasma membrane aquaporin regulation.The Plant cell · 2011Article
- Early germination of Arabidopsis pollen in a double null mutant for the arabinogalactan protein genes AGP6 and AGP11.Sexual plant reproduction · 2010Article
- Under pressure, cell walls set the pace.Trends in plant science · 2010Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
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Abstract
To investigate the role of aquaporin-mediated water transport during pollen grain germination and tube growth, Arabidopsis thaliana plasma membrane intrinsic proteins (PIPs) were expressed in pollen of Lilium longiflorum (lily). Successful expression of AtPIPs in particle-bombarded lily pollen grains was monitored by co-expression with fluorescent proteins and single-cell RT-PCR, and by measuring the water permeability coefficient (P(os)) in swelling assays using protoplasts prepared from transformed pollen grains and tubes. Expression of AtPIP1;1 and AtPIP1;2 in pollen grains resulted in P(os) values similar to those measured in nontransformed pollen grain protoplasts (6.65 +/- 2.41 microm s(-1)), whereas expression of AtPIP2 significantly increased P(os) (AtPIP2;1, 13.79 +/- 6.38; AtPIP2;2, 10.16 +/- 3.30 microm s(-1)). Transformation with combinations of AtPIP1 and AtPIP2 did not further enhance P(os). Native pollen tube protoplasts showed higher P(os) values (13.23 +/- 4.14 microm s(-1)) than pollen grain protoplasts but expression of AtPIP2;1 (18.85 +/- 7.60 microm s(-1)) did not significantly increase their P(os) values. Expression of none of the tested PIPs had any effect on pollen tube growth rates. The ectopic expression of AtPIP2s in lily pollen increased the water permeability of the plasma membrane in pollen grains, but not in pollen tubes. The measured endogenous water permeability does not limit water uptake during tube growth, but has to be regulated to prevent tube bursting.
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