ArticleMolecular and cellular endocrinology2008
NeuroD1 and Mash1 temporally regulate GnRH receptor gene expression in immortalized mouse gonadotrope cells.
Article in Molecular and cellular endocrinology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Recent Advances in the Understanding of Gonadotrope Lineage Differentiation in the Developing Pituitary.Neuroendocrinology · 2025Review
- Article
- Pituitary Tumors and Immortalized Cell Lines Generated by Cre-Inducible Expression of SV40 T Antigen.Endocrinology · 2021Article
- Androgen receptor positively regulates gonadotropin-releasing hormone receptor in pituitary gonadotropes.Molecular and cellular endocrinology · 2021Article
- Conservation of Three-Dimensional Helix-Loop-Helix Structure through the Vertebrate Lineage Reopens the Cold Case of Gonadotropin-Releasing Hormone-Associated Peptide.Frontiers in endocrinology · 2017Article
- Homeodomain Proteins SIX3 and SIX6 Regulate Gonadotrope-specific Genes During Pituitary Development.Molecular endocrinology (Baltimore, Md.) · 2015Article
- Msx1 homeodomain protein represses the αGSU and GnRH receptor genes during gonadotrope development.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Pituitary gland development and disease: from stem cell to hormone production.Current topics in developmental biology · 2013Review
- Targeted pituitary overexpression of pituitary adenylate-cyclase activating polypeptide alters postnatal sexual maturation in male mice.Endocrinology · 2012Article
- Mechanisms underlying the tissue-specific and regulated activity of the Gnrhr promoter in mammals.Frontiers in endocrinology · 2012Article
- Hypothalamic dysregulation and infertility in mice lacking the homeodomain protein Six6.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Molecular mechanisms of pituitary organogenesis: In search of novel regulatory genes.Molecular and cellular endocrinology · 2010Review
- Runt-related transcription factors impair activin induction of the follicle-stimulating hormone {beta}-subunit gene.Endocrinology · 2010Article
- Otx2 induction of the gonadotropin-releasing hormone promoter is modulated by direct interactions with Grg co-repressors.The Journal of biological chemistry · 2009Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Accurate spatial and temporal expression of gonadotrope-specific genes, such as the gonadotropin-releasing hormone receptor (GnRHR) gene, is critical for gonadotrope maturation. Herein, we show that a specific E-box in the mouse GnRHR promoter binds two group A basic-helix-loop-helix (bHLH) transcription factors. Mutation of this E-box decreases expression in mouse gonadotrope-derived alphaT3-1 and LbetaT2 cell lines. Microarray and western blots show that the bHLH transcription factor NeuroD1 is strongly expressed in the gonadotrope progenitor, alphaT3-1, whereas Mash1 is strongly expressed in the more mature gonadotrope, LbetaT2. Over-expression of NeuroD1 or Mash1 increases expression of the GnRHR gene or a multimer of the E-box and this increase is lost upon mutation of the E-box. Electrophoretic mobility shift assays reveal that the GnRHR E-box binds NeuroD1 from alphaT3-1 cells, but binds Mash1 from LbetaT2 cells. The sequential binding of different members of the group A bHLH transcription factor family to mouse GnRHR E-box 3 as the gonadotrope differentiates may represent a mechanism necessary for proper spatial and temporal expression of the GnRHR during gonadotrope development.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.