ArticleBMC immunology2008
Primer sets for cloning the human repertoire of T cell Receptor Variable regions.
Article in BMC immunology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
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- Identification of a shared antigen linking CD4Science advances · 2026Article
- Massively Scalable Single-cell Multiomic Profiling of T Cell Repertoire with REFLEX.Research square · 2025Article
- REFLEX, a Novel Immune Profiling Assay, Combining TCR Repertoire and Multiome at Massively Scalable Single-cell Resolution to Catapult Exploration of T-cell Derived Immunity.bioRxiv : the preprint server for biology · 2025Article
- NY-ESO-1-specific T cell receptor-engineered T cells and Tranilast, a TRPV2 antagonist bivalent treatment enhances the killing of esophageal cancer: a dual-targeted cancer therapeutic route.Cancer cell international · 2024Article
- Cell activation-based screening of natively paired human T cell receptor repertoires.Scientific reports · 2023Article
- Identification of TRDV-TRAJ V domains in human and mouse T-cell receptor repertoires.Frontiers in immunology · 2023Article
- BCG-activation of leukocytes is sufficient for the generation of donor-independent innate anti-tumor NK and γδ T-cells that can be further expandedOncoimmunology · 2023Article
- Immortalization and functional screening of natively paired human T cell receptor repertoires.Protein engineering, design & selection : PEDS · 2022Article
- A bicistronic vector backbone for rapid seamless cloning and chimerization of αβT-cell receptor sequences.PloS one · 2020Article
- Escherichiacoli-Specific CD4+ T Cells Have Public T-Cell Receptors and Low Interleukin 10 Production in Crohn's Disease.Cellular and molecular gastroenterology and hepatology · 2020Observational
- Recent Advances in Targeting CD8 T-Cell Immunity for More Effective Cancer Immunotherapy.Frontiers in immunology · 2018Review
- A Unique Subset of γδ T Cells Expands and Produces IL-10 in Patients with Naturally Acquired Immunity against Falciparum Malaria.Frontiers in microbiology · 2017Article
- Engineered cytotoxic T lymphocytes with AFP-specific TCR gene for adoptive immunotherapy in hepatocellular carcinoma.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Article
- Discovery of invariant T cells by next-generation sequencing of the human TCR α-chain repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Broad T-cell receptor repertoire in T-lymphocytes derived from human induced pluripotent stem cells.PloS one · 2014Article
- The important role of T cells and receptor expression in Sjögren's syndrome.Scandinavian journal of immunology · 2013Review
- How (specific) would like your T-cells today? Generating T-cell therapeutic function through TCR-gene transfer.Frontiers in immunology · 2012Article
- Article
- Profiling the T-cell receptor beta-chain repertoire by massively parallel sequencing.Genome research · 2009Article
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAmplification and cloning of naïve T cell Receptor (TR) repertoires or antigen-specific TR is crucial to shape immune response and to develop immuno-based therapies. TR variable (V) regions are encoded by several genes that recombine during T cell development. The cloning of expressed genes as large diverse libraries from natural sources relies upon the availability of primers able to amplify as many V genes as possible.
resultsHere, we present a list of primers computationally designed on all functional TR V and J genes listed in the IMGT, the ImMunoGeneTics information system. The list consists of unambiguous or degenerate primers suitable to theoretically amplify and clone the entire TR repertoire. We show that it is possible to selectively amplify and clone expressed TR V genes in one single RT-PCR step and from as little as 1000 cells.
conclusionThis new primer set will facilitate the creation of more diverse TR libraries than has been possible using currently available primer sets.
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