ArticleArthritis research & therapy2008
Induction of HLA-B27 heavy chain homodimer formation after activation in dendritic cells.
Article in Arthritis research & therapy, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Conformational Alterations of the Cell Surface of Monomeric and Dimeric β2m-Free HLA-I (Proto-HLA) May Enable Novel Immune Functions in Health and Disease.Current issues in molecular biology · 2024Review
- Cell Surface B2m-Free Human Leukocyte Antigen (HLA) Monomers and Dimers: Are They Neo-HLA Class and Proto-HLA?Biomolecules · 2023Review
- Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease Progression in Rheumatoid Arthritis Patients under Treatment.Antibodies (Basel, Switzerland) · 2023Article
- Four Faces of Cell-Surface HLA Class-I: Their Antigenic and Immunogenic Divergence Generating Novel Targets for Vaccines.Vaccines · 2022Review
- Transcriptome-Wide Association Studies and Integration Analysis of mRNA Expression Profiles Identify Candidate Genes and Pathways Associated With Ankylosing Spondylitis.Frontiers in immunology · 2022Article
- Allelic association with ankylosing spondylitis fails to correlate with human leukocyte antigen B27 homodimer formation.The Journal of biological chemistry · 2019Article
- Dendritic cells in the pathogenesis of ankylosing spondylitis and axial spondyloarthritis.Clinical rheumatology · 2019Review
- Quantitative proteomic changes in LPS-activated monocyte-derived dendritic cells: A SWATH-MS study.Scientific reports · 2019Article
- An HLA-B27 Homodimer Specific Antibody Recognizes a Discontinuous Mixed-Disulfide Epitope as Identified by Affinity-Mass Spectrometry.Journal of the American Society for Mass Spectrometry · 2016Article
- Divide, Conquer, and Sense: CD8Frontiers in immunology · 2016Article
- Article
- HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.PloS one · 2015Article
- Injectable MMP-sensitive alginate hydrogels as hMSC delivery systems.Biomacromolecules · 2014Article
- Article
- MHC class I dimer formation by alteration of the cellular redox environment and induction of apoptosis.Immunology · 2012Article
- Expression of MHC class I dimers and ERAP1 in an ankylosing spondylitis patient cohort.Immunology · 2011Article
- Pathogenicity of Misfolded and Dimeric HLA-B27 Molecules.International journal of rheumatology · 2011Article
- Progress in spondylarthritis. Immunopathogenesis of spondyloarthritis: which cells drive disease?Arthritis research & therapy · 2009Review
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
introductionAnkylosing spondylitis (AS) is a severe, chronic inflammatory arthritis, with a strong association to the human major histocompatibilty complex (MHC) class I allele human leucocyte antigen (HLA) B27. Disulfide-linked HLA-B27 heavy-chain homodimers have been implicated as novel structures involved in the aetiology of AS. We have studied the formation of HLA-B27 heavy-chain homodimers in human dendritic cells, which are key antigen-presenting cells and regulators of mammalian immune responses.
methodBoth an in vitro dendritic-like cell line and monocyte-derived dendritic cells from peripheral blood were studied. The KG-1 dendritic-like cell line was transfected with HLA-B27 cDNA constructs, and the cellular distribution, intracellular assembly and ability of HLA-B27 to form heavy-chain homodimers was compared with human monocyte-derived dendritic cells after stimulation with bacterial lipopolysaccharide (LPS).
resultsImmature KG-1 cells expressing HLA-B27 display an intracellular source of MHC class I heavy-chain homodimers partially overlapping with the Golgi bodies, but not the endoplasmic reticulum, which is lost at cell maturation with phorbyl-12-myristate-13-acetate (PMA) and ionomycin. Significantly, the formation of HLA-B27 homodimers in transfected KG-1 cells is induced by maturation, with a transient induction also seen in LPS-stimulated human monocyte-derived dendritic cells expressing HLA-B27. The weak association of wildtype HLA-B*2705 with the transporter associated with antigen processing could also be enhanced by mutation of residues at position 114 and 116 in the peptide-binding groove to those present in the HLA-B*2706 allele.
conclusionWe have demonstrated that HLA-B27 heavy-chain homodimer formation can be induced by dendritic cell activation, implying that these novel structures may not be displayed to the immune system at all times. Our data suggests that the behaviour of HLA-B27 on dendritic cells may be important in the study of inflammatory arthritis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.