ArticleTissue engineering. Part A2009
Screening for 3D environments that support human mesenchymal stem cell viability using hydrogel arrays.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 77 citations in OpenAlex.
- Engineering a 3D Biomimetic Peptides Functionalized-Polyethylene Glycol Hydrogel Model Cocultured with Endothelial Cells and Astrocytes: Enhancing In Vitro Blood-Brain Barrier Biomimicry.Molecular pharmaceutics · 2024Article
- Serum-Free Cultures: Could They Be a Future Direction to Improve Neuronal Differentiation of Mesenchymal Stromal Cells?International journal of molecular sciences · 2022Review
- Engineering the Dynamics of Cell Adhesion Cues in Supramolecular Hydrogels for Facile Control over Cell Encapsulation and Behavior.Advanced materials (Deerfield Beach, Fla.) · 2021Article
- A glance on the role of fibronectin in controlling cell response at biomaterial interface.The Japanese dental science review · 2020Review
- Engineered Perineural Vascular Plexus for Modeling Developmental Toxicity.Advanced healthcare materials · 2020Article
- Screening a chemically defined extracellular matrix mimetic substrate library to identify substrates that enhance substrate-mediated transfection.Experimental biology and medicine (Maywood, N.J.) · 2020Article
- Mesenchymal Stem/Progenitor Cells: The Prospect of Human Clinical Translation.Stem cells international · 2020Review
- Rapid Diels-Alder Cross-linking of Cell Encapsulating Hydrogels.Chemistry of materials : a publication of the American Chemical Society · 2019Article
- Engineering Hydrogel Microenvironments to Recapitulate the Stem Cell Niche.Annual review of biomedical engineering · 2018Review
- RGD-presenting peptides in amphiphilic and anionic β-sheet hydrogels for improved interactions with cells.RSC advances · 2018Article
- Spreading, proliferation and differentiation of human dental pulp stem cells on chitosan scaffolds immobilized with RGD or fibronectin.Cytotechnology · 2017Article
- Article
- Tyrosine-Selective Functionalization for Bio-Orthogonal Cross-Linking of Engineered Protein Hydrogels.Bioconjugate chemistry · 2017Article
- Combinatorial Method/High Throughput Strategies for Hydrogel Optimization in Tissue Engineering Applications.Gels (Basel, Switzerland) · 2016Review
- Bio-Orthogonally Crosslinked, Engineered Protein Hydrogels with Tunable Mechanics and Biochemistry for Cell Encapsulation.Advanced functional materials · 2016Article
- Hydrogel arrays formed via differential wettability patterning enable combinatorial screening of stem cell behavior.Acta biomaterialia · 2016Article
- Characterization of the crosslinking kinetics of multi-arm poly(ethylene glycol) hydrogels formed via Michael-type addition.Soft matter · 2016Article
- Improving Cell Engraftment in Cardiac Stem Cell Therapy.Stem cells international · 2016Review
- Materials as stem cell regulators.Nature materials · 2014Review
- IKVAV regulates ERK1/2 and Akt signalling pathways in BMMSC population growth and proliferation.Cell proliferation · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
In this study we generated 3D poly(ethylene glycol) (PEG) hydrogel arrays to screen for the individual and combinatorial effects of extracellular matrix (ECM) degradability, cell adhesion ligand type, and cell adhesion ligand density on human mesenchymal stem cell (hMSC) viability. In particular, we explored the influence of two well-characterized ECM-derived cell adhesion ligands: the fibronectin-derived Arg-Gly-Asp-Ser-Pro (RGDSP) sequence, and the laminin-derived Ile-Lys-Val-Ala-Val (IKVAV) sequence. PEG network degradation, the RGDSP ligand, and the IKVAV ligand each individually increased hMSC viability in a dose-dependent manner. The RGDSP ligand also improved hMSC viability in a dose-dependent manner in degradable PEG hydrogels, while the effect of IKVAV was less pronounced in degradable hydrogels. Combinations of RGDSP and IKVAV promoted high viability of hMSCs in nondegradable PEG networks, while the combined effects of the ligands were not significant in degradable PEG hydrogels. Although hMSC spreading was not commonly observed within PEG hydrogels, we qualitatively observed hMSC spreading after 5 days only in degradable PEG hydrogels prepared with 2.5 mM of both RGDSP and IKVAV. These results suggest that the enhanced throughput approach described herein can be used to rapidly study the influence of a broad range of ECM parameters, as well as their combinations, on stem cell behavior.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.