ArticleTissue engineering. Part A2009
Insulin-like growth factor-I and slow, bi-directional perfusion enhance the formation of tissue-engineered cardiac grafts.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 59 citations in OpenAlex.
- Microfluidic Chamber Design for Organ-on-a-Chip: A Computational Fluid Dynamics Study of Pillar Geometry and Pulsatile Perfusion.Biosensors · 2026Article
- Bridging the Gap: Advances and Challenges in Heart Regeneration from In Vitro to In Vivo Applications.Bioengineering (Basel, Switzerland) · 2024Review
- Comparative Analysis of Heart Regeneration: Searching for the Key to Heal the Heart-Part II: Molecular Mechanisms of Cardiac Regeneration.Journal of cardiovascular development and disease · 2023Review
- Independent, Controllable Stretch-Perfusion Bioreactor Chambers to Functionalize Cell-Seeded Decellularized Tendons.Annals of biomedical engineering · 2020Article
- Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality.Stem cell reports · 2019Article
- Expansion Culture of Human Pluripotent Stem Cells and Production of Cardiomyocytes.Bioengineering (Basel, Switzerland) · 2019Review
- Enhancing all-in-one bioreactors by combining interstitial perfusion, electrical stimulation, on-line monitoring and testing within a single chamber for cardiac constructs.Scientific reports · 2018Article
- Bone regeneration with osteogenic matrix cell sheet and tricalcium phosphate: An experimental study in sheep.World journal of orthopedics · 2017Article
- Developmental stage-dependent effects of cardiac fibroblasts on function of stem cell-derived engineered cardiac tissues.Scientific reports · 2017Article
- Article
- Tissue engineering the cardiac microenvironment: Multicellular microphysiological systems for drug screening.Advanced drug delivery reviews · 2016Review
- Article
- PI3K Phosphorylation Is Linked to Improved Electrical Excitability in an In Vitro Engineered Heart Tissue Disease Model System.Tissue engineering. Part A · 2015Article
- Establishing Early Functional Perfusion and Structure in Tissue Engineered Cardiac Constructs.Critical reviews in biomedical engineering · 2015Review
- Use of flow, electrical, and mechanical stimulation to promote engineering of striated muscles.Annals of biomedical engineering · 2014Review
- Biomimetic scaffold combined with electrical stimulation and growth factor promotes tissue engineered cardiac development.Experimental cell research · 2014Article
- Three-dimensional elastomeric scaffolds designed with cardiac-mimetic structural and mechanical features.Tissue engineering. Part A · 2013Article
- Biomimetic perfusion and electrical stimulation applied in concert improved the assembly of engineered cardiac tissue.Journal of tissue engineering and regenerative medicine · 2012Article
- Pluripotent stem cell-derived cardiac tissue patch with advanced structure and function.Biomaterials · 2011Article
- The significance of pore microarchitecture in a multi-layered elastomeric scaffold for contractile cardiac muscle constructs.Biomaterials · 2011Article
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Authors and funding
4 authors at 2 institutions in 1 country.
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Abstract
Biochemical and mechanical signals enabling cardiac regeneration can be elucidated using in vitro tissue-engineering models. We hypothesized that insulin-like growth factor-I (IGF) and slow, bi-directional perfusion could act independently and interactively to enhance the survival, differentiation, and contractile performance of tissue-engineered cardiac grafts. Heart cells were cultured on three-dimensional porous scaffolds in medium with or without supplemental IGF and in the presence or absence of slow, bi-directional perfusion that enhanced transport and provided shear stress. Structural, molecular, and electrophysiologic properties of the resulting grafts were quantified on culture day 8. IGF had independent, beneficial effects on apoptosis (p < 0.01), cellular viability (p < 0.01), contractile amplitude (p < 0.01), and excitation threshold (p < 0.01). Perfusion independently affected the four aforementioned parameters and also increased amounts of cardiac troponin-I (p < 0.01), connexin-43 (p < 0.05), and total protein (p < 0.01) in the grafts. Interactive effects of IGF and perfusion on apoptosis were also present (p < 0.01). Myofibrillogenesis and spontaneous contractility were present only in grafts cultured with perfusion, although contractility was inducible by electrical field stimulation of grafts from all groups. Our findings demonstrate that multi-factorial stimulation of tissue-engineered cardiac grafts using IGF and perfusion resulted in independent and interactive effects on heart cell survival, differentiation, and contractility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.