ArticleTissue engineering. Part A2009
Engineering endochondral bone: in vitro studies.
Article in Tissue engineering. Part A, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Article
- Orthotopic Bone Formation by Streamlined Engineering and Devitalization of Human Hypertrophic Cartilage.International journal of molecular sciences · 2020Article
- Biomaterial-based endochondral bone regeneration: a shift from traditional tissue engineering paradigms to developmentally inspired strategies.Materials today. Bio · 2019Review
- High-density human mesenchymal stem cell rings with spatiotemporally-controlled morphogen presentation as building blocks for engineering bone diaphyseal tissue.Nanotheranostics · 2018Article
- Effects of in vitro endochondral priming and pre-vascularisation of human MSC cellular aggregates in vivo.Stem cell research & therapy · 2015Article
- Endochondral ossification for enhancing bone regeneration: converging native extracellular matrix biomaterials and developmental engineering in vivo.Tissue engineering. Part B, Reviews · 2015Review
- Finding and tracing human MSC in 3D microenvironments with the photoconvertible protein Dendra2.Scientific reports · 2015Article
- Formation of cartilage and synovial tissue by human gingival stem cells.Stem cells and development · 2014Article
- Stem cell-derived endochondral cartilage stimulates bone healing by tissue transformation.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2014Article
- Novel 4-arm poly(ethylene glycol)-block-poly(anhydride-esters) amphiphilic copolymer micelles loading curcumin: preparation, characterization, and in vitro evaluation.BioMed research international · 2013Article
- Biomedical Applications of Biodegradable Polymers.Journal of polymer science. Part B, Polymer physics · 2011Article
- In-vivo generation of bone via endochondral ossification by in-vitro chondrogenic priming of adult human and rat mesenchymal stem cells.BMC musculoskeletal disorders · 2011Article
- F-spondin regulates chondrocyte terminal differentiation and endochondral bone formation.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2010Article
- Cytokine expression and accelerated tooth movement.Journal of dental research · 2010Article
- An improved collagen scaffold for skeletal regeneration.Journal of biomedical materials research. Part A · 2010Article
- Vascular endothelial growth factor inhibits bone morphogenetic protein 2 expression in rat mesenchymal stem cells.Tissue engineering. Part A · 2010Article
- CORRELATION BETWEEN MICRO-CT SECTIONS AND HISTOLOGICAL SECTIONS OF MOUSE SKULL DEFECTS IMPLANTED WITH ENGINEERED CARTILAGE.Gravitational and space biology bulletin : publication of the American Society for Gravitational and Space Biology · 2009Article
- Engineering endochondral bone: in vivo studies.Tissue engineering. Part A · 2009Article
- Bone defect reconstruction via endochondral ossification: A developmental engineering strategy.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Chitosan scaffolds have been shown to possess biological and mechanical properties suitable for tissue engineering and clinical applications. In the present work, chitosan sponges were evaluated regarding their ability to support cartilage cell proliferation and maturation, which are the first steps in endochondral bone formation. Chitosan sponges were seeded with chondrocytes isolated from chicken embryo sterna. Chondrocyte/chitosan constructs were cultured for 20 days, and treated with retinoic acid (RA) to induce chondrocyte maturation and matrix synthesis. At different time points, samples were collected for microscopic, histological, biochemical, and mechanical analyses. Results show chondrocyte attachment, proliferation, and abundant matrix synthesis, completely obliterating the pores of the sponges. RA treatment caused chondrocyte hypertrophy, characterized by the presence of type X collagen in the extracellular matrix and increased alkaline phosphatase activity. In addition, hypertrophy markedly changed the mechanical properties of the chondrocyte/chitosan constructs. In conclusion, we have developed chitosan sponges with adequate pore structure and mechanical properties to serve as a support for hypertrophic chondrocytes. In parallel studies, we have evaluated the ability of this mature cartilage scaffold to induce endochondral ossification.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.