ArticleMolecular and cellular biology1991
Identification of a specific exon sequence that is a major determinant in the selection between a natural and a cryptic 5' splice site.
Article in Molecular and cellular biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 44 citations in OpenAlex.
- Structural and Functional RNA Motifs of SARS-CoV-2 and Influenza A Virus as a Target of Viral Inhibitors.International journal of molecular sciences · 2023Review
- The influenza A segment 7 mRNA 3' splice site pseudoknot/hairpin family.RNA biology · 2012Article
- The 3' splice site of influenza A segment 7 mRNA can exist in two conformations: a pseudoknot and a hairpin.PloS one · 2012Article
- Role of RNA structure in regulating pre-mRNA splicing.Trends in biochemical sciences · 2010Review
- Modulating role of RNA structure in alternative splicing of a critical exon in the spinal muscular atrophy genes.Nucleic acids research · 2007Article
- Cryptic splice site usage in exon 7 of the human fibrinogen Bbeta-chain gene is regulated by a naturally silent SF2/ASF binding site within this exon.RNA (New York, N.Y.) · 2006Article
- Defective splicing, disease and therapy: searching for master checkpoints in exon definition.Nucleic acids research · 2006Review
- 5'- and 3'-terminal nucleotides in the FGFR2 ISAR splicing element core have overlapping roles in exon IIIb activation and exon IIIc repression.Nucleic acids research · 2001Article
- Regulation of fibronectin EDA exon alternative splicing: possible role of RNA secondary structure for enhancer display.Molecular and cellular biology · 1999Article
- Multiple interdependent sequence elements control splicing of a fibroblast growth factor receptor 2 alternative exon.Molecular and cellular biology · 1997Article
- Presence of negative and positive cis-acting RNA splicing elements within and flanking the first tat coding exon of human immunodeficiency virus type 1.Molecular and cellular biology · 1994Article
- Splice site selection in polyomavirus late pre-mRNA processing.Journal of virology · 1994Article
- Two different sequence elements within exon 4 are necessary for calcitonin-specific splicing of the human calcitonin/calcitonin gene-related peptide I pre-mRNA.Molecular and cellular biology · 1994Article
- Upstream and downstream cis-acting elements for cleavage at the L4 polyadenylation site of adenovirus-2.Nucleic acids research · 1994Article
- Short artificial hairpins sequester splicing signals and inhibit yeast pre-mRNA splicing.Molecular and cellular biology · 1993Article
- The cardiac troponin T alternative exon contains a novel purine-rich positive splicing element.Molecular and cellular biology · 1993Article
- Domains within the mammalian ornithine decarboxylase messenger RNA have evolved independently and episodically.Journal of molecular evolution · 1993Article
- Factors affecting authentic 5' splice site selection in plant nuclei.Molecular and cellular biology · 1993Article
- Selection between a natural and a cryptic 5' splice site: a kinetic study of the effect of upstream exon sequences.Gene expression · 1993Article
- cis-acting sequences involved in exon selection in the chicken beta-tropomyosin gene.Molecular and cellular biology · 1992Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first intron of the early region 3 from adenovirus type 2 contains a cryptic 5' splice site, Dcr1, 74 nucleotides downstream from the natural site D1. The cryptic site can be activated when the natural site is inactivated by mutagenesis. To investigate the basis for selection between a natural and a cryptic 5' splice site, we searched for cis-acting elements responsible for the exclusive selection of the natural site. We show that both the relative intrinsic strength of the sites and the sequence context affect the selection. A 120-nucleotide segment located at the 3' end of exon 1 enhances splicing at the proximal site D1; in its absence the two sites are used according to their strength. Thus, three cis-acting elements are involved in the silencing of the cryptic site: the sequence of D1, the sequence of Dcr1, and an upstream exonic sequence. We show that the exonic element folds, in solution, into a 113-nucleotide-long stem-loop structure. We propose that this potential stem-loop structure which is located 6 nucleotides upstream of the exon 1-intron junction is responsible for the preferential use of the natural 5' splice site.
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