ArticleArthritis and rheumatism2008
Rho kinase-dependent CCL20 induced by dynamic compression of human chondrocytes.
Article in Arthritis and rheumatism, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 47 citations in OpenAlex.
- Recent advances and emerging mechanisms in graphene-based materials for cartilage tissue engineering.npj biomedical innovations · 2026Review
- Role of external forces in the mechanobiology of stem and differentiated chondrogenic cells embedded in a tissue-engineered construct for cartilage repair.Journal of biomedical science · 2026Review
- Compressive stress-driven Piezo1 activation and Rho-ROCK mechanotransduction promote tumor progression via epigenetic mechanical memory.Science advances · 2026Article
- Mechanical cues enhance chondrocyte function: Insights from mechanoreception, regulation, and biological responses.Mechanobiology in medicine · 2025Review
- Migration and homeostasis of regulatory T cells in rheumatoid arthritis.Frontiers in immunology · 2022Review
- Insulin-like growth factor-1 regulates the mechanosensitivity of chondrocytes by modulating TRPV4.Cell calcium · 2021Article
- Inflammatory signaling sensitizes Piezo1 mechanotransduction in articular chondrocytes as a pathogenic feed-forward mechanism in osteoarthritis.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Role of Chemokines and Chemokine Receptors in Rheumatoid Arthritis.ImmunoTargets and therapy · 2020Review
- Primary human chondrocytes respond to compression with phosphoproteomic signatures that include microtubule activation.Journal of biomechanics · 2019Article
- Relationship between serum and synovial fluid CCL20 concentrations with disease severity in primary knee osteoarthritis.Journal of musculoskeletal & neuronal interactions · 2019Article
- Tendinosis develops from age- and oxygen tension-dependent modulation of Rac1 activity.Aging cell · 2019Article
- RhoA/ROCK pathway: implication in osteoarthritis and therapeutic targets.American journal of translational research · 2019Review
- The Multifunctional Role of the Chemokine System in Arthritogenic Processes.Current rheumatology reports · 2017Review
- Candidate mediators of chondrocyte mechanotransduction via targeted and untargeted metabolomic measurements.Archives of biochemistry and biophysics · 2014Article
- Wnt and Rho GTPase signaling in osteoarthritis development and intervention: implications for diagnosis and therapy.Arthritis research & therapy · 2013Review
- Differential activation and inhibition of RhoA by fluid flow induced shear stress in chondrocytes.Cell biology international · 2013Article
- Cyclic compression-induced p38 activation and subsequent MMP13 expression requires Rho/ROCK activity in bovine cartilage explants.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2012Article
- Effects of perfusion and dynamic loading on human neocartilage formation in alginate hydrogels.Tissue engineering. Part A · 2012Article
- Th17 cells expressing KIR3DL2+ and responsive to HLA-B27 homodimers are increased in ankylosing spondylitis.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- Vimentin contributes to changes in chondrocyte stiffness in osteoarthritis.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2011Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
objectiveMechanical stimulation of cartilage affects tissue homeostasis and chondrocyte function. The chondrocyte phenotype is dependent on cell shape, which is largely determined by the actin cytoskeleton. Reorganization of the actin cytoskeleton results from Rho GTPase activation. The purpose of this study was to examine the roles of both actin and Rho in mechanotransduction in chondrocytes.
methodsWe embedded human articular chondrocytes in 2 x 6-mm agarose discs at 5 x 10(6) cells/ml and subjected the discs to unconfined dynamic compression at 0.5 Hz. By comparing samples with and without dynamic compression, we identified Rho activation according to the GTP-bound active RhoA measured in cell lysates. We identified rearrangements in filamentous actin structures using fluorescence-labeled phalloidin and confocal microscopy of fixed samples. We identified altered gene expression using TaqMan quantitative reverse transcription-polymerase chain reaction analysis. We tested for a requirement for Rho signaling by performing the dynamic compression in the presence of Rho kinase inhibitors.
resultsRhoA activation occurred within 5-10 minutes of dynamic compression. Rho kinase-dependent actin reorganization occurred within 20 minutes after application of dynamic compression and was apparent as "punctate" F-actin structures that were visible under confocal microscopy. We identified early-phase mechanoresponsive genes (CCL20 and inducible nitric oxide synthase) that were highly up-regulated within 1 hour of dynamic compression in a Rho kinase-dependent and actin-dependent manner.
conclusionTogether, these results are the first demonstration that the Rho-Rho kinase pathway and actin cytoskeletal reorganization are required for changes in the expression of genes involved in human chondrocyte mechanotransduction.
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