ArticleGlycoconjugate journal2009
Chemo-enzymatic synthesis of poly-N-acetyllactosamine (poly-LacNAc) structures and their characterization for CGL2-galectin-mediated binding of ECM glycoproteins to biomaterial surfaces.
Article in Glycoconjugate journal, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 80 citations in OpenAlex.
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- Glycan synthesis with SpyCatcher-SpyTag immobilized Leloir-glycosyltransferases.Applied microbiology and biotechnology · 2025Article
- Fractionation of Oligosaccharide Nucleoside Mixtures by Single Pass Nano-Diafiltration.Engineering in life sciences · 2025Article
- Human Milk Oligosaccharides Multivalently Presented on Defined Synthetic Neo-Glycoproteins Are Nanomolar Ligands of Tandem-Repeat Galectins.Biomacromolecules · 2025Article
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- Selective Glycopolymer Inhibitors of Galectin-3: Supportive Anti-Cancer Agents Protecting Monocytes and Preserving Interferon-Gamma Function.International journal of nanomedicine · 2025Article
- Impacts of β-1, 3-N-acetylglucosaminyltransferases (B3GNTs) in human diseases.Molecular biology reports · 2024Review
- Site-Specific N- and O-Glycosylation Analysis of Human Plasma Fibronectin.Frontiers in chemistry · 2021Article
- Cell-free systems for accelerating glycoprotein expression and biomanufacturing.Journal of industrial microbiology & biotechnology · 2020Review
- Enzymatic Cascades for TailoredMolecules (Basel, Switzerland) · 2019Article
- The β-Molecules (Basel, Switzerland) · 2019Article
- Chemoenzymatic Synthesis of Galectin Binding Glycopolymers.Bioconjugate chemistry · 2018Article
- Machine-Driven Enzymatic Oligosaccharide Synthesis by Using a Peptide Synthesizer.Angewandte Chemie (International ed. in English) · 2018Article
- Poly-N-Acetyllactosamine Neo-Glycoproteins as Nanomolar Ligands of Human Galectin-3: Binding Kinetics and Modeling.International journal of molecular sciences · 2018Article
- A Compartmented Flow Microreactor System for Automated Optimization of Bioprocesses Applying Immobilized Enzymes.Frontiers in bioengineering and biotechnology · 2018Article
- Enzymatic Synthesis of N-Acetyllactosamine (LacNAc) Type 1 Oligomers and Characterization as Multivalent Galectin Ligands.Molecules (Basel, Switzerland) · 2017Article
- Identification of the binding roles of terminal and internal glycan epitopes using enzymatically synthesized N-glycans containing tandem epitopes.Organic & biomolecular chemistry · 2016Article
- Sequential One-Pot Multienzyme Chemoenzymatic Synthesis of Glycosphingolipid Glycans.The Journal of organic chemistry · 2016Article
- Donor substrate promiscuity of bacterial β1-3-N-acetylglucosaminyltransferases and acceptor substrate flexibility of β1-4-galactosyltransferases.Bioorganic & medicinal chemistry · 2016Article
- Microwave-Assisted Synthesis of Glycoconjugates by Transgalactosylation with Recombinant Thermostable β-Glycosidase from Pyrococcus.International journal of molecular sciences · 2016Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Poly-N-acetyllactosamine (poly-LacNAc) structures have been identified as important ligands for galectin-mediated cell adhesion to extra-cellular matrix (ECM) proteins. We here present the biofunctionalization of surfaces with poly-LacNAc structures and subsequent binding of ECM glycoproteins. First, we synthesized beta-GlcNAc glycosides carrying a linker for controlled coupling onto chemically functionalized surfaces. Then we produced poly-LacNAc structures with defined lengths using human beta1,4-galactosyltransferase-1 and beta1,3-N-acetylglucosaminyltransferase from Helicobacter pylori. These compounds were also used for kinetic characterization of glycosyltransferases and lectin binding assays. A mixture of poly-LacNAc-structures covalently coupled to functionalized microtiter plates were identified for best binding to our model galectin His(6)CGL2. We further demonstrate for the first time that these poly-LacNAc surfaces are suitable for further galectin-mediated binding of the ECM glycoproteins laminin and fibronectin. This new technology should facilitate cell adhesion to biofunctionalized surfaces by imitating the natural ECM microenvironment.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.