ArticlePlant cell reports2008
Efficient production of genetically engineered, male-sterile Arabidopsis thaliana using anther-specific promoters and genes derived from Brassica oleracea and B. rapa.
Article in Plant cell reports, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 40 citations in OpenAlex.
- Male sterility in plants: an overview of advancements from natural CMS to genetically manipulated systems for hybrid seed production.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Review
- A proteomic study of cysteine protease induced cell death in anthers of male sterile tobacco transgenic plants.Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Development of a visible marker trait based on leaf sheath-specific anthocyanin pigmentation applicable to various genotypes in rice.Breeding science · 2019Article
- Tapetal Expression ofFrontiers in plant science · 2019Article
- Development of transgenic male-sterile rice by using anther-specific promoters identified by comprehensive screening of the gene expression profile database 'RiceXPro'.Breeding science · 2018Article
- Development and characterization of transgenic dominant male sterile rice toward an outcross-based breeding system.Breeding science · 2018Article
- Evolvement of transgenic male-sterility and fertility-restoration system in rice for production of hybrid varieties.Plant molecular biology · 2018Article
- Functional architecture of two exclusively late stage pollen-specific promoters in rice (Oryza sativa L.).Plant molecular biology · 2015Article
- A novel male sterility-fertility restoration system in plants for hybrid seed production.Scientific reports · 2015Article
- Expression of a pathogen-induced cysteine protease (AdCP) in tapetum results in male sterility in transgenic tobacco.Functional & integrative genomics · 2014Article
- Article
- The promoter of an A9 homolog from the conifer Cryptomeria japonica imparts male strobilus-dominant expression in transgenic trees.Plant cell reports · 2013Article
- Spatial and temporal activity of upstream regulatory regions of rice anther-specific genes in transgenic rice and Arabidopsis.Transgenic research · 2013Article
- Comprehensive analysis of genic male sterility-related genes in Brassica rapa using a newly developed Br300K oligomeric chip.PloS one · 2013Article
- Agronomic traits and gene containment capability of cleistogamous rice lines with the superwoman1-cleistogamy mutation.Breeding science · 2012Article
- Silencing of meiosis-critical genes for engineering male sterility in plants.Plant cell reports · 2012Article
- Sustainable use of biotechnology for bioenergy feedstocks.Environmental management · 2010Article
- Article
- A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus: the role of abscisic acid in early anther development.Plant molecular biology · 2010Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Prevention of transgene flow from genetically modified crops to food crops and wild relatives is of concern in agricultural biotechnology. We used genes derived from food crops to produce complete male sterility as a strategy for gene confinement as well as to reduce the food purity concerns of consumers. Anther-specific promoters (A3, A6, A9, MS2, and MS5) were isolated from Brassica oleracea and B. rapa and fused to the beta-glucuronidase (GUS) reporter gene and candidate genes for male sterility, including the cysteine proteases BoCysP1 and BoCP3, and negative regulatory components of phytohormonal responses involved in male development. These constructs were then introduced into Arabidopsis thaliana. GUS analyses revealed that A3, A6, and A9 had tapetum-specific promoter activity from the anther meiocyte stage. Male sterility was confirmed in tested constructs with protease or gibberellin insensitive (gai) genes. In particular, constructs with BoCysP1 driven by the A3 or A9 promoter most efficiently produced plants with complete male sterility. The tapetum and middle layer cells of anthers expressing BoCysP1 were swollen and excessively vacuolated when observed in transverse section. This suggests that the ectopic expression of cysteine protease in the meiocyte stage may inhibit programmed cell death. The gai gene also induced male sterility, although at a low frequency. This is the first report to show that plant cysteine proteases and gai from food crops are available as a novel tool for the development of genetically engineered male-sterile plants.
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