ArticleApplied microbiology and biotechnology2008
Production of 2-methyl-1-butanol in engineered Escherichia coli.
Article in Applied microbiology and biotechnology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 142 citations in OpenAlex.
- Tile-Based Fisher Ratio Analysis with Support Vector Machine Regression Modeling of GC×GC-TOFMS Data of VOCs Produced byAnalytical chemistry · 2025Article
- Biosynthesis pathways of expanding carbon chains for producing advanced biofuels.Biotechnology for biofuels and bioproducts · 2023Review
- High-Level Production of Isoleucine and Fusel Alcohol by Expression of the Feedback Inhibition-Insensitive Threonine Deaminase inApplied and environmental microbiology · 2022Article
- Article
- Enriching the Production of 2-Methyl-1-Butanol in Fermentation Process Using Corynebacterium crenatum.Current microbiology · 2020Article
- Establishment of BmoR-based biosensor to screen isobutanol overproducer.Microbial cell factories · 2019Article
- Biosynthesis, regulation, and engineering of microbially produced branched biofuels.Biotechnology for biofuels · 2019Review
- Production efficiency of the bacterial non-ribosomal peptide indigoidine relies on the respiratory metabolic state in S. cerevisiae.Microbial cell factories · 2018Article
- Identifying metabolic elements that contribute to productivity of 1-propanol bioproduction using metabolomic analysis.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Amino acid catabolism-directed biofuel production inBiotechnology reports (Amsterdam, Netherlands) · 2017Review
- Engineering the leucine biosynthetic pathway for isoamyl alcohol overproduction in Saccharomyces cerevisiae.Journal of industrial microbiology & biotechnology · 2017Article
- Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae.Scientific reports · 2016Article
- Systems Metabolic Engineering of Escherichia coli.EcoSal Plus · 2016Review
- Converting Sugars to Biofuels: Ethanol and Beyond.Bioengineering (Basel, Switzerland) · 2015Review
- 2-Keto acids based biosynthesis pathways for renewable fuels and chemicals.Journal of industrial microbiology & biotechnology · 2015Review
- Metabolic engineering of microbes for branched-chain biodiesel production with low-temperature property.Biotechnology for biofuels · 2015Article
- Review
- Structure-guided engineering of Lactococcus lactis alcohol dehydrogenase LlAdhA for improved conversion of isobutyraldehyde to isobutanol.Journal of biotechnology · 2012Article
- Biomass production from electricity using ammonia as an electron carrier in a reverse microbial fuel cell.PloS one · 2012Article
- Recent progress in synthetic biology for microbial production of C3-C10 alcohols.Frontiers in microbiology · 2012Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recent progress has been made in the production of higher alcohols by harnessing the power of natural amino acid biosynthetic pathways. Here, we describe the first strain of Escherichia coli developed to produce the higher alcohol and potential new biofuel 2-methyl-1-butanol (2MB). To accomplish this, we explored the biodiversity of enzymes catalyzing key parts of the isoleucine biosynthetic pathway, finding that AHAS II (ilvGM) from Salmonella typhimurium and threonine deaminase (ilvA) from Corynebacterium glutamicum improve 2MB production the most. Overexpression of the native threonine biosynthetic operon (thrABC) on plasmid without the native transcription regulation also improved 2MB production in E. coli. Finally, we knocked out competing pathways upstream of threonine production (DeltametA, Deltatdh) to increase its availability for further improvement of 2MB production. This work led to a strain of E. coli that produces 1.25 g/L 2MB in 24 h, a total alcohol content of 3 g/L, and with yields of up to 0.17 g 2MB/g glucose.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.