ArticleGenetics2008
Genetic basis of evolutionary adaptation by Escherichia coli to stressful cycles of freezing, thawing and growth.
Article in Genetics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 45 citations in OpenAlex.
- Isolation and Characterization of StrainMicroorganisms · 2021Article
- Cryopreservation of clonal and polyclonal populations ofBiology methods & protocols · 2021Article
- Consequences of Cryopreservation in Diverse Natural Isolates of Saccharomyces cerevisiae.Genome biology and evolution · 2020Article
- Domestication of Campylobacter jejuni NCTC 11168.Microbial genomics · 2019Article
- Experimental Design, Population Dynamics, and Diversity in Microbial Experimental Evolution.Microbiology and molecular biology reviews : MMBR · 2018Review
- Development of Freeze-Thaw TolerantFrontiers in microbiology · 2018Article
- Stress-Induced Evolution of Heat Resistance and Resuscitation Speed in Escherichia coli O157:H7 ATCC 43888.Applied and environmental microbiology · 2016Article
- How Archiving by Freezing Affects the Genome-Scale Diversity of Escherichia coli Populations.Genome biology and evolution · 2016Article
- Different tradeoffs result from alternate genetic adaptations to a common environment.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Metagenomic analysis of size-fractionated picoplankton in a marine oxygen minimum zone.The ISME journal · 2014Article
- Adaptive laboratory evolution -- principles and applications for biotechnology.Microbial cell factories · 2013Review
- Rationally designed bidirectional promoter improves the evolutionary stability of synthetic genetic circuits.Nucleic acids research · 2013Article
- Evolutionary potential, cross-stress behavior and the genetic basis of acquired stress resistance in Escherichia coli.Molecular systems biology · 2013Article
- Role of rpoS in the development of cell envelope resilience and pressure resistance in stationary-phase Escherichia coli.Applied and environmental microbiology · 2011Article
- Insertion sequence-driven evolution of Escherichia coli in chemostats.Journal of molecular evolution · 2011Article
- Designing and engineering evolutionary robust genetic circuits.Journal of biological engineering · 2010Article
- Molecular and evolutionary bases of within-patient genotypic and phenotypic diversity in Escherichia coli extraintestinal infections.PLoS pathogens · 2010Article
- Reduced evolvability of Escherichia coli MDS42, an IS-less cellular chassis for molecular and synthetic biology applications.Microbial cell factories · 2010Article
- Cryptic lineages of the genus Escherichia.Applied and environmental microbiology · 2009Article
- Molecular genetic approaches to defining lipid function.Journal of lipid research · 2009Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microbial evolution experiments offer a powerful approach for coupling changes in complex phenotypes, including fitness and its components, with specific mutations. Here we investigate mutations substituted in 15 lines of Escherichia coli that evolved for 1000 generations under freeze-thaw-growth (FTG) conditions. To investigate the genetic basis of their improvements, we screened many of the lines for mutations involving insertion sequence (IS) elements and identified two genes where multiple lines had similar mutations. Three lines had IS150 insertions in cls, which encodes cardiolipin synthase, and 8 lines had IS150 insertions in the uspA-uspB intergenic region, encoding two universal stress proteins. Another line had an 11-bp deletion mutation in the cls gene. Strain reconstructions and competitions demonstrated that this deletion is beneficial under the FTG regime in its evolved genetic background. Further experiments showed that this cls mutation helps maintain membrane fluidity after freezing and thawing and improves freeze-thaw (FT) survival. Reconstruction of isogenic strains also showed that the IS150 insertions in uspA/B are beneficial under the FTG regime. The evolved insertions reduce uspB transcription and increase both FT survival and recovery, but the physiological mechanism for this fitness improvement remains unknown.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.