ArticleMicrobiology (Reading, England)2008
The Salmonella SPI-2 effector SseJ exhibits eukaryotic activator-dependent phospholipase A and glycerophospholipid : cholesterol acyltransferase activity.
Article in Microbiology (Reading, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed, 75 citations in OpenAlex.
- The Manipulation of Host Cells byInternational journal of molecular sciences · 2026Review
- The interplay betweenCell insight · 2025Review
- Review
- Eeukaryotic-like Sppsk1 from Vibrio splendidus AJ01 mediates phagosome escape via inhibiting phagosome acidification and maturation.Cellular and molecular life sciences : CMLS · 2025Article
- Controlling intracellular protein delivery, tumor colonization and tissue distribution using flhDC in clinically relevant ΔsseJ Salmonella.Molecular therapy : the journal of the American Society of Gene Therapy · 2025Article
- Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus.Communications biology · 2024Article
- Paired single-cell host profiling with multiplex-tagged bacterial mutants reveals intracellular virulence-immune networks.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Speaking the host language: howMicrobiology (Reading, England) · 2023Review
- VpdC is a ubiquitin-activated phospholipase effector that regulatesProceedings of the National Academy of Sciences of the United States of America · 2022Article
- The phospholipase A effector PlaA from Legionella pneumophila: expression, purification and crystallization.Acta crystallographica. Section F, Structural biology communications · 2020Article
- SopF, a phosphoinositide binding effector, promotes the stability of the nascent Salmonella-containing vacuole.PLoS pathogens · 2019Article
- The Role of the Type III Secretion System in the Intracellular Lifestyle of Enteric Pathogens.Microbiology spectrum · 2019Review
- Pathways of host cell exit by intracellular pathogens.Microbial cell (Graz, Austria) · 2018Review
- Disulfide loop cleavage of Legionella pneumophila PlaA boosts lysophospholipase A activity.Scientific reports · 2017Article
- Article
- Galectin-3 directs antimicrobial guanylate binding proteins to vacuoles furnished with bacterial secretion systems.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- What the SIF Is Happening-The Role of IntracellularFrontiers in cellular and infection microbiology · 2017Review
- The Salmonella effector SseJ disrupts microtubule dynamics when ectopically expressed in normal rat kidney cells.PloS one · 2017Article
- How Bacteria Subvert Animal Cell Structure and Function.Annual review of cell and developmental biology · 2016Review
- Bacterial Sphingomyelinases and Phospholipases as Virulence Factors.Microbiology and molecular biology reviews : MMBR · 2016Review
Corrections and comments
- Commented on by
Authors and funding
5 authors at 1 institution in 2 countries.
Funding
Abstract
Intracellular replication of Salmonella enterica serovar Typhimurium within membrane-bound compartments, called Salmonella-containing vacuoles, depends on the activities of several effector proteins translocated by the Salmonella pathogenicity island 2 (SPI-2)-encoded type III secretion system. The SPI-2 effector protein SseJ shows similarity at the amino acid level to several GDSL lipases with glycerophospholipid : cholesterol acyltransferase (GCAT) activity. In this study, we show that catalytic serine-dependent phospholipase A (PLA) and GCAT activity of recombinant SseJ is potentiated by factor(s) present in HeLa cells, RAW macrophages and Saccharomyces cerevisiae. SseJ activity was enhanced with increasing amounts of, or preincubation with, eukaryotic cell extracts. Analysis of the activating factor(s) shows that it is soluble and heat- and protease-sensitive. We conclude that PLA and GCAT activities of SseJ are potentiated by proteinaceous eukaryotic factor(s).
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.