ArticleApplied and environmental microbiology2008
Functional expression and subcellular localization of the aflatoxin pathway enzyme Ver-1 fused to enhanced green fluorescent protein.
Article in Applied and environmental microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed, 44 citations in OpenAlex.
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- Targeting of Specialized Metabolites Biosynthetic Enzymes to Membranes and Vesicles by Posttranslational Palmitoylation: A Mechanism of Non-Conventional Traffic and Secretion of Fungal Metabolites.International journal of molecular sciences · 2024Review
- Vacuole Proteins with Optimized Microtubule Assembly Is Required for Fum1 Protein Localization and Fumonisin Biosynthesis in Mycotoxigenic FungusJournal of fungi (Basel, Switzerland) · 2023Article
- Subcellular localization of fungal specialized metabolites.Fungal biology and biotechnology · 2022Review
- A novel phosphoinositide kinase Fab1 regulates biosynthesis of pathogenic aflatoxin inVirulence · 2021Article
- FgVps9, a Rab5 GEF, Is Critical for DON Biosynthesis and Pathogenicity inFrontiers in microbiology · 2020Article
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- The Fungal bZIP Transcription Factor AtfB Controls Virulence-Associated Processes in Aspergillus parasiticus.Toxins · 2017Article
- Functional Genomic Analysis of Aspergillus flavus Interacting with Resistant and Susceptible Peanut.Toxins · 2016Article
- SNARE protein FgVam7 controls growth, asexual and sexual development, and plant infection in Fusarium graminearum.Molecular plant pathology · 2016Article
- Cellular compartmentalization of secondary metabolism.Frontiers in microbiology · 2015Review
- Biodiversity of genes encoding anti-microbial traits within plant associated microbes.Frontiers in plant science · 2015Review
- Spatial and temporal control of fungal natural product synthesis.Natural product reports · 2014Review
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- Cellular development associated with induced mycotoxin synthesis in the filamentous fungus Fusarium graminearum.PloS one · 2013Article
- Proteomic and biochemical evidence support a role for transport vesicles and endosomes in stress response and secondary metabolism in Aspergillus parasiticus.Journal of proteome research · 2012Article
- Two novel classes of enzymes are required for the biosynthesis of aurofusarin in Fusarium graminearum.The Journal of biological chemistry · 2011Article
- Compartmentalization and molecular traffic in secondary metabolism: a new understanding of established cellular processes.Fungal genetics and biology : FG & B · 2011Review
Corrections and comments
- Erratum issued
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Aflatoxin, a mycotoxin synthesized by Aspergillus spp., is among the most potent naturally occurring carcinogens known. Little is known about the subcellular organization of aflatoxin synthesis. Previously, we used transmission electron microscopy and immunogold labeling to demonstrate that the late aflatoxin enzyme OmtA localizes primarily to vacuoles in fungal cells on the substrate surface of colonies. In the present work, we monitored subcellular localization of Ver-1 in real time in living cells. Aspergillus parasiticus strain CS10-N2 was transformed with plasmid constructs that express enhanced green fluorescent protein (EGFP) fused to Ver-1. Analysis of transformants demonstrated that EGFP fused to Ver-1 at either the N or C terminus functionally complemented nonfunctional Ver-1 in recipient cells. Western blot analysis detected predominantly full-length Ver-1 fusion proteins in transformants. Confocal laser scanning microscopy demonstrated that Ver-1 fusion proteins localized in the cytoplasm and in the lumen of up to 80% of the vacuoles in hyphae grown for 48 h on solid media. Control EGFP (no Ver-1) expressed in transformants was observed in only 13% of the vacuoles at this time. These data support a model in which middle and late aflatoxin enzymes are synthesized in the cytoplasm and transported to vacuoles, where they participate in aflatoxin synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.