ArticleBiophysical journal2008
Measurement of instantaneous velocity vectors of organelle transport: mitochondrial transport and bioenergetics in hippocampal neurons.
Article in Biophysical journal, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 48 citations in OpenAlex.
- Carbon-to-ATP ratios across the kingdoms of life.iScience · 2026Article
- Latest assessment methods for mitochondrial homeostasis in cognitive diseases.Neural regeneration research · 2024Review
- Visualization Method for the Cell-Level Vesicle Transport Using Optical Flow and a Diverging Colormap.Sensors (Basel, Switzerland) · 2021Article
- Monitoring and Determining Mitochondrial Network Parameters in Live Lung Cancer Cells.Journal of clinical medicine · 2019Article
- Chronic Energy Depletion due to Iron Deficiency Impairs Dendritic Mitochondrial Motility during Hippocampal Neuron Development.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Article
- Article
- Resistive flow sensing of vital mitochondria with nanoelectrodes.Mitochondrion · 2017Article
- Article
- Dynamics of Mitochondrial Transport in Axons.Frontiers in cellular neuroscience · 2016Article
- Automated detection of whole-cell mitochondrial motility and its dependence on cytoarchitectural integrity.Biotechnology and bioengineering · 2015Article
- Automated measurement of fast mitochondrial transport in neurons.Frontiers in cellular neuroscience · 2015Article
- No consistent bioenergetic defects in presynaptic nerve terminals isolated from mouse models of Alzheimer's disease.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2012Article
- Quantitative measurement of mitochondrial membrane potential in cultured cells: calcium-induced de- and hyperpolarization of neuronal mitochondria.The Journal of physiology · 2012Article
- Mitochondrial dynamics: the intersection of form and function.Advances in experimental medicine and biology · 2012Review
- The parkinsonian mimetic, MPP+, specifically impairs mitochondrial transport in dopamine axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Intrinsic bioenergetic properties and stress sensitivity of dopaminergic synaptosomes.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2011Article
- Biophysical properties of mitochondrial fusion events in pancreatic beta-cells and cardiac cells unravel potential control mechanisms of its selectivity.American journal of physiology. Cell physiology · 2010Article
- Organellar vs cellular control of mitochondrial dynamics.Seminars in cell & developmental biology · 2010Review
- Tickled PINK1: mitochondrial homeostasis and autophagy in recessive Parkinsonism.Biochimica et biophysica acta · 2010Review
- Mitochondria in cardiomyocyte Ca2+ signaling.The international journal of biochemistry & cell biology · 2009Review
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
Impaired transport of mitochondria, in dendrites and axons of neurons, and bioenergetic deficit are increasingly recognized to be of pathological importance in neurodegenerative diseases. To study the relationship between transport and bioenergetics, we have developed what to our knowledge is a novel technique to quantify organelle velocity in cultured cells. The aim was to combine measurement of motion and bioenergetic parameters while minimizing photodynamic oxidative artifacts evoked by fluorescence excitation. Velocity determination from sequential fluorescence images is not trivial, and here we describe an application of "optical flow", the flow of gray values in grayscale images, to this problem. Based on the principles of photon shot noise occurring in low light level fluorescence microscopy, we describe and validate here an optical flow-based, robust method to measure velocity vectors for organelles expressing fluorescent proteins. This method features instantaneous velocity determination from a pair of images by detecting motion of edges, with no assumptions about the separation or shapes of the objects in the image. Optical flow was used in combination with single mitochondrion assay of mitochondrial thiol redox status by mitochondrially targeted redox-sensitive green fluorescent protein and measurement of mitochondrial membrane potential by tetramethylrhodamine methyl ester. Mitochondrial populations of resting cultured hippocampal neurons were analyzed. It was found that mitochondria with more oxidized thiol redox status have lower membrane potentials and are smaller in size. These mitochondria are more motile than the average; however, mitochondrial motility is only slightly dependent on the observed bioenergetic parameters and is correlated the best to the size of the mitochondria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.