ArticleJournal of bacteriology2008
Regulation by nucleoid-associated proteins at the Escherichia coli nir operon promoter.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 38 citations in OpenAlex.
- Phaseolotoxin: Environmental Conditions and Regulatory Mechanisms Involved in Its Synthesis.Microorganisms · 2024Review
- The morphology and metabolic changes of Actinobacillus pleuropneumoniae during its growth as a biofilm.Veterinary research · 2023Article
- Novel organisation and regulation of theVirulence · 2022Article
- Regulation of nrf operon expression in pathogenic enteric bacteria: sequence divergence reveals new regulatory complexity.Molecular microbiology · 2017Article
- Local and global regulation of transcription initiation in bacteria.Nature reviews. Microbiology · 2016Review
- Silencing of DNase Colicin E8 Gene Expression by a Complex Nucleoprotein Assembly Ensures Timely Colicin Induction.PLoS genetics · 2015Article
- Molecular dissection of Mycobacterium tuberculosis integration host factor reveals novel insights into the mode of DNA binding and nucleoid compaction.The Journal of biological chemistry · 2014Article
- Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS in attaching and effacing Escherichia coli.Journal of bacteriology · 2012Article
- Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12.Nucleic acids research · 2012Article
- Direct and indirect effects of H-NS and Fis on global gene expression control in Escherichia coli.Nucleic acids research · 2011Article
- Repression of aerobic leukotoxin transcription by integration host factor in Aggregatibacter actinomycetemcomitans.Research in microbiology · 2010Article
- Down-regulation of the Escherichia coli K-12 nrf promoter by binding of the NsrR nitric oxide-sensing transcription repressor to an upstream site.Journal of bacteriology · 2010Article
- Mechanisms and evolution of control logic in prokaryotic transcriptional regulation.Microbiology and molecular biology reviews : MMBR · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The Escherichia coli K-12 nir operon promoter can be fully activated by binding of the regulator of fumarate and nitrate reduction (FNR) to a site centered at position -41.5 upstream of the transcript start, and this activation is modulated by upstream binding of the integration host factor (IHF) and Fis (factor for inversion stimulation) proteins. Thus, transcription initiation is repressed by the binding of IHF and Fis to sites centered at position -88 (IHF I) and position -142 (Fis I) and activated by IHF binding to a site at position -115 (IHF II). Here, we have exploited mutational analysis and biochemistry to investigate the actions of IHF and Fis at these sites. We show that the effects of IHF and Fis are position dependent and that IHF II functions independently of IHF I and Fis I. Using in vitro assays, we report that IHF and Fis repress transcription initiation by interfering with RNA polymerase binding. Differences in the upstream IHF and Fis binding sites at the nir promoter in related enteric bacteria fix the level of nir operon expression under anaerobic growth conditions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.