ArticleJournal of bacteriology2008
Characterization of the Caulobacter crescentus holdfast polysaccharide biosynthesis pathway reveals significant redundancy in the initiating glycosyltransferase and polymerase steps.
Article in Journal of bacteriology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 85 citations in OpenAlex.
- CauloKO: an ordered transposon mutant library inJournal of bacteriology · 2026Article
- Identification of EcpK, a bacterial tyrosine pseudokinase important for exopolysaccharide biosynthesis inJournal of bacteriology · 2025Article
- Unipolar Polysaccharide-mediated Attachment of the NMicrobes and environments · 2025Article
- Coaggregation of oral pathogens by postbiotic lactobacilli.Journal of oral microbiology · 2025Article
- An exopolysaccharide pathway from a freshwaterJournal of bacteriology · 2024Article
- A novel exopolysaccharide pathway from a freshwaterbioRxiv : the preprint server for biology · 2023Article
- Sticky decisions: The multilayered regulation of adhesin production by bacteria.PLoS genetics · 2023Article
- HfaE Is a Component of the Holdfast Anchor Complex That Tethers the Holdfast Adhesin to the Cell Envelope.Journal of bacteriology · 2022Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Caulobacter lipid A is conditionally dispensable in the absence of fur and in the presence of anionic sphingolipids.Cell reports · 2022Article
- Dual adhesive unipolar polysaccharides synthesized by overlapping biosynthetic pathways in Agrobacterium tumefaciens.Molecular microbiology · 2022Article
- Surface sensing stimulates cellular differentiation inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion.PLoS biology · 2020Article
- Comparative Analysis of Ionic Strength Tolerance between Freshwater and MarineJournal of bacteriology · 2019Article
- A Multiprotein Complex Anchors Adhesive Holdfast at the Outer Membrane of Caulobacter crescentus.Journal of bacteriology · 2019Article
- Role ofJournal of bacteriology · 2019Article
- Composition of the Holdfast Polysaccharide fromJournal of bacteriology · 2019Article
- Regulation of bacterial surface attachment by a network of sensory transduction proteins.PLoS genetics · 2019Article
- A Genome-Wide Analysis of Adhesion inmBio · 2019Article
- Genome-scale fitness profile of Caulobacter crescentus grown in natural freshwater.The ISME journal · 2019Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Caulobacter crescentus cells adhere to surfaces by using an extremely strong polar adhesin called the holdfast. The polysaccharide component of the holdfast is comprised in part of oligomers of N-acetylglucosamine. The genes involved in the export of the holdfast polysaccharide and the anchoring of the holdfast to the cell were previously discovered. In this study, we identified a cluster of polysaccharide biosynthesis genes (hfsEFGH) directly adjacent to the holdfast polysaccharide export genes. Sequence analysis indicated that these genes are involved in the biosynthesis of the minimum repeat unit of the holdfast polysaccharide. HfsE is predicted to be a UDP-sugar lipid-carrier transferase, the glycosyltransferase that catalyzes the first step in polysaccharide biosynthesis. HfsF is predicted to be a flippase, HfsG is a glycosyltransferase, and HfsH is similar to a polysaccharide (chitin) deacetylase. In-frame hfsG and hfsH deletion mutants resulted in severe deficiencies both in surface adhesion and in binding to the holdfast-specific lectin wheat germ agglutinin. In contrast, hfsE and hfsF mutants exhibited nearly wild-type levels of adhesion and holdfast synthesis. We identified three paralogs to hfsE, two of which are redundant to hfsE for holdfast synthesis. We also identified a redundant paralog to the hfsC gene, encoding the putative polysaccharide polymerase, and present evidence that the hfsE and hfsC paralogs, together with the hfs genes, are absolutely required for proper holdfast synthesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.