ArticleInvestigative ophthalmology & visual science2009
Role of cell and matrix-bound VEGF isoforms in lens development.
Article in Investigative ophthalmology & visual science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed, 1 synthesis or guideline pooled it, 23 citations in OpenAlex.
- Pooled it
- VEGF188 promotes corneal reinnervation after injury.JCI insight · 2019Article
- Effects of anti-vascular endothelial growth factor monoclonal antibody (bevacizumab) on lens epithelial cells.Clinical ophthalmology (Auckland, N.Z.) · 2016Article
- Geographic Atrophy and Choroidal Neovascularization in the Same Eye: A Review.Ophthalmic research · 2016Review
- Cooperative effects of FGF-2 and VEGF-A in periodontal ligament cells.Journal of dental research · 2014Article
- Dynamic and differential regulation in the microRNA expression in the developing and mature cataractous rat lens.Journal of cellular and molecular medicine · 2013Article
- Lenticular cytoprotection. Part 1: the role of hypoxia inducible factors-1α and -2α and vascular endothelial growth factor in lens epithelial cell survival in hypoxia.Molecular vision · 2013Article
- αA-crystallin and αB-crystallin reside in separate subcellular compartments in the developing ocular lens.The Journal of biological chemistry · 2012Article
- Frs2α enhances fibroblast growth factor-mediated survival and differentiation in lens development.Development (Cambridge, England) · 2012Article
- Expression and role of VEGF--a in the ciliary body.Investigative ophthalmology & visual science · 2012Article
- Capsular Block Syndrome after an Intravitreal Injection of Ranibizumab: A Case Report.Case reports in ophthalmologyArticle
- VEGF and VEGFR2 bind to similar pH-sensitive sites on fibronectin, exposed by heparin-mediated conformational changes.The Journal of biological chemistryArticle
- The function of vascular endothelial growth factor.BioFactors (Oxford, England)Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeTo determine the role of vascular endothelial growth factor (VEGF) in embryonic eye development and lens differentiation.
methodsExpression of components of the VEGF signaling pathway during lens development and in adults was characterized by beta-galactosidase staining of VEGF-LacZ mice, immunohistochemistry, and real-time (q) PCR. Embryonic eyes from wild-type mice and VEGF120/120 mice were analyzed by light microscopy and immunohistochemistry. VEGF function during lens development was analyzed using eye explants treated with VEGF-neutralizing antibody. Direct function of VEGF was demonstrated on the human lens epithelial cell line, HLE-B3.
resultsEmbryonic lens epithelium and posterior lens fibers expressed VEGF and VEGFR2. qPCR revealed VEGF164 as the major isoform in embryonic lens. Transgenic mice expressing only VEGF120 (VEGF120/120 mice) showed major defects in eye development, including microphthalmia, failed lens differentiation, and hyperplastic hyaloid vessels. The lens displayed abnormal cell patterning and differentiation associated with altered c-Maf, Prox1, and p57 expression pattern in the anterior epithelium. The number of proliferating epithelial cells was drastically reduced in VEGF120/120 lenses. Altered MIP26 cellular localization and reduced E-cadherin expression in the lens epithelium were observed. VEGF-neutralization led to reduced fiber elongation of eye explants. Exogenous VEGF increased survival and proliferation of HLE-B3 cell in a dose-dependent manner.
conclusionsAbnormalities in ocular development in VEGF120/120 mice suggest a role for VEGF not only in the formation of ocular vascular beds but also in the differentiation of the lens itself.
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Registered trials
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