ArticleThe Journal of physiology2008
Internalized Kv1.5 traffics via Rab-dependent pathways.
Article in The Journal of physiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Rab10 plays a protective role in the development of pathological cardiac hypertrophy.Journal of molecular and cellular cardiology plus · 2025Article
- Subcellular trafficking and endocytic recycling of KAmerican journal of physiology. Cell physiology · 2022Review
- KInternational journal of molecular sciences · 2021Article
- Review
- Statin-specific inhibition of Rab-GTPase regulates cPKC-mediated IKs internalization.Scientific reports · 2019Article
- Fluvastatin inhibits Rab5-mediated IKs internalization caused by chronic CaJournal of molecular and cellular cardiology · 2019Article
- Modulation of TMEM16A channel activity by the von Willebrand factor type A (VWA) domain of the calcium-activated chloride channel regulator 1 (CLCA1).The Journal of biological chemistry · 2017Article
- Distinct subcellular mechanisms for the enhancement of the surface membrane expression of SK2 channel by its interacting proteins, α-actinin2 and filamin A.The Journal of physiology · 2017Article
- Angiotensin II reduces the surface abundance of KThe Journal of physiology · 2017Article
- Rab11-dependent Recycling of the Human Ether-a-go-go-related Gene (hERG) Channel.The Journal of biological chemistry · 2015Article
- Intravascular pressure enhances the abundance of functional Kv1.5 channels at the surface of arterial smooth muscle cells.Science signaling · 2015Article
- Shear stress triggers insertion of voltage-gated potassium channels from intracellular compartments in atrial myocytes.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Oestrogen promotes KCNQ1 potassium channel endocytosis and postendocytic trafficking in colonic epithelium.The Journal of physiology · 2013Article
- Rab5 regulates internalisation of P2X4 receptors and potentiation by ivermectin.Purinergic signalling · 2013Article
- Trafficking of an endogenous potassium channel in adult ventricular myocytes.American journal of physiology. Cell physiology · 2012Article
- Novel molecular targets for atrial fibrillation therapy.Nature reviews. Drug discovery · 2012Review
- Disruption of kv1.3 channel forward vesicular trafficking by hypoxia in human T lymphocytes.The Journal of biological chemistry · 2012Article
- Autophagy is constitutively active in normal mouse sino-atrial nodal cells.Acta histochemica et cytochemica · 2011Article
- Antidepressant-induced ubiquitination and degradation of the cardiac potassium channel hERG.The Journal of biological chemistry · 2011Article
- Rab11 supports amphetamine-stimulated norepinephrine transporter trafficking.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2010Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the postinternalization trafficking of surface-expressed voltage-gated potassium channels. Here, for the first time, we investigate into which of four major trafficking pathways a voltage-gated potassium channel is targeted after internalization. In both a cardiac myoblast cell line and in HEK293 cells, channels were found to internalize and to recycle quickly. Upon internalization, Kv1.5 rapidly associated with Rab5-and Rab4-positive endosomes, suggesting that the channel is internalized via a Rab5-dependent pathway and rapidly targeted for recycling to the plasma membrane. Nevertheless, as indicated by colocalization with Rab7, a fraction of the channels are targeted for degradation. Recycling through perinuclear endosomes is limited; colocalization with Rab11 was evident only after 24 h postsurface labelling. Expression of dominant negative (DN) Rab constructs significantly increased Kv1.5 functional expression. In the myoblast line, Rab5DN increased Kv1.5 current densities to 1305 +/- 213 pA pF(-1) from control 675 +/- 81.6 pA pF(-1). Rab4DN similarly increased Kv1.5 currents to 1382 +/- 155 pA pF(-1) from the control 522 +/- 82.7 pA pF(-1) at +80 mV. Expression of the Rab7DN increased Kv1.5 currents 2.5-fold in HEK293 cells but had no significant effect in H9c2 myoblasts, and, unlike the other Rab GTPases tested, over-expression of wild-type Rab7 decreased Kv1.5 currents in the myoblast line. Densities fell to 573 +/- 96.3 pA pF(-1) from the control 869 +/- 135.5 pA pF(-1). The Rab11DN was slow to affect Kv1.5 currents but had comparable effects to other dominant negative constructs after 48 h. With the exception of Rab11DN and nocodazole, the effects of interference with microtubule-dependent trafficking by nocodazole or p50 overexpression were not additive with the Rab dominant negatives. The Rab GTPases thus constitute dynamic targets by which cells may modulate Kv1.5 functional expression.
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