ArticleThe Journal of biological chemistry2008
Arabidopsis phosphomannose isomerase 1, but not phosphomannose isomerase 2, is essential for ascorbic acid biosynthesis.
Article in The Journal of biological chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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48 citing papers in PubMed, 101 citations in OpenAlex.
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- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Antioxidants of Non-Enzymatic Nature: Their Function in Higher Plant Cells and the Ways of Boosting Their Biosynthesis.Antioxidants (Basel, Switzerland) · 2023Review
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Genetic and biochemical strategies for regulation of L-ascorbic acid biosynthesis in plants through the L-galactose pathway.Frontiers in plant science · 2023Review
- Glucomannan inGenes · 2022Review
- Comparative Analysis of Antioxidant Accumulation under Cold Acclimation, Deacclimation and Reacclimation in Winter Wheat.Plants (Basel, Switzerland) · 2022Article
- Identification, Characteristics and Function of Phosphoglucomutase (PGM) in the Agar Biosynthesis and Carbon Flux in the AgarophyteMarine drugs · 2022Article
- Metabolism and Regulation of Ascorbic Acid in Fruits.Plants (Basel, Switzerland) · 2022Review
- GWAS on multiple traits identifies mitochondrial ACONITASE3 as important for acclimation to submergence stress.Plant physiology · 2022Article
- Mobile forms of carbon in trees: metabolism and transport.Tree physiology · 2022Review
- Exogenous brassinolide improves the antioxidant capacity ofFrontiers in plant science · 2022Article
- Analysis of Ascorbate Metabolism in Arabidopsis Under High-Light Stress.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Development of a Genome-Edited Tomato With High Ascorbate Content During Later Stage of Fruit Ripening Through Mutation ofFrontiers in plant science · 2022Article
- Transcriptomics Reveals Host-Dependent Differences of Polysaccharides Biosynthesis inMolecules (Basel, Switzerland) · 2021Article
- Transcriptomic Changes in Internode Explants of Stinging Nettle during Callogenesis.International journal of molecular sciences · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- Characterization of an algal phosphomannose isomerase gene and its application as a selectable marker for genetic manipulation of tomato.Plant diversity · 2021Article
- Three metabolic pathways are responsible for the accumulation and maintenance of high AsA content in kiwifruit (Actinidia eriantha).BMC genomics · 2021Article
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Authors and funding
6 authors at 3 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
We studied molecular and functional properties of Arabidopsis phosphomannose isomerase isoenzymes (PMI1 and PMI2) that catalyze reversible isomerization between D-fructose 6-phosphate and D-mannose 6-phosphate (Man-6P). The apparent K(m) and V(max) values for Man-6P of purified recombinant PMI1 were 41.3+/-4.2 microm and 1.89 micromol/min/mg protein, respectively, whereas those of purified recombinant PMI2 were 372+/-13 microm and 22.5 micromol/min/mg protein, respectively. Both PMI1 and PMI2 were inhibited by incubation with EDTA, Zn(2+), Cd(2+), and L-ascorbic acid (AsA). Arabidopsis PMI1 protein was constitutively expressed in both vegetative and reproductive organs under normal growth conditions, whereas the PMI2 protein was not expressed in any organs under light. The induction of PMI1 expression and an increase in the AsA level were observed in leaves under continuous light, whereas the induction of PMI2 expression and a decrease in the AsA level were observed under long term darkness. PMI1 showed a diurnal expression pattern in parallel with the total PMI activity and the total AsA content in leaves. Moreover, a reduction of PMI1 expression through RNA interference resulted in a substantial decrease in the total AsA content of leaves of knockdown PMI1 plants, whereas the complete inhibition of PMI2 expression did not affect the total AsA levels in leaves of knock-out PMI2 plants. Consequently, this study improves our understanding of the molecular and functional properties of Arabidopsis PMI isoenzymes and provides genetic evidence of the involvement of PMI1, but not PMI2, in the biosynthesis of AsA in Arabidopsis plants.
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