ArticleBMC research notes2008
Differential susceptibility of PCR reactions to inhibitors: an important and unrecognised phenomenon.
Article in BMC research notes, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 110 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
110 citing papers in PubMed, 242 citations in OpenAlex.
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- Use of External Quality Control Material for HIV-1 RNA Testing To Assess the Comparability of Data Generated in Separate Laboratories and the Stability of HIV-1 RNA in Samples after Prolonged Storage.Journal of clinical microbiology · 2018Trial
- Comparative evaluation of two high-throughput qPCR systems for environmental and clinical microbiology applications.Applied and environmental microbiology · 2026Article
- Antimicrobial resistance in wastewater-impacted coastal waters: implications for environmental surveillance and public health.Microbiology (Reading, England) · 2026Article
- Performance assessment of a duplex quantitative PCR assay for detectingMicrobiology spectrum · 2026Article
- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.Applied and environmental microbiology · 2026Article
- Detection of Certain Berries in Difficult Samples by Singleplex and Multiplex Real-Time PCR-HRM: A Case Study of Pitfalls.Methods and protocols · 2026Article
- Comparative Evaluation of Rapid Nucleic Acids Extraction Methods for Biosensor-Based Point-of-Care Solutions.Biosensors · 2026Article
- Sensitivity of Loop-Mediated Isothermal Amplification in Comparison to Digital Droplet PCR for Identification ofFoods (Basel, Switzerland) · 2026Article
- Dietary DNA Metabarcoding From Animal Fecal Samples.Current protocols · 2026Article
- Analysis of qPCR Data: From PCR Efficiency to Absolute Target Quantity.International journal of molecular sciences · 2025Review
- Adaptation of the multiplexed CRISPR-Cas13 CARMEN RVP assay for longitudinal detection of respiratory pathogens from air samples.medRxiv : the preprint server for health sciences · 2025Article
- When Two-Fold Is Not Enough: Quantifying Uncertainty in Low-Copy qPCR.International journal of molecular sciences · 2025Article
- Skin biopsy processing for rapid molecular diagnosis and histopathologic interpretation: application to Kaposi sarcoma in East Africa.Infectious agents and cancer · 2025Article
- MIQE 2.0 and the Urgent Need to Rethink qPCR Standards.International journal of molecular sciences · 2025Article
- The Impact of Viral Concentration Method on Quantification and Long Amplicon Nanopore Sequencing of SARS-CoV-2 and Noroviruses in Wastewater.Microorganisms · 2025Article
- Exploring the Pathogen Profiles of Ancient Feces.bioRxiv : the preprint server for biology · 2025Article
- Targeted pathogen profiling of ancient feces reveals common enteric infections in the Rio Zape Valley, 725-920 CE.PloS one · 2025Article
- Dataset for a validated method of non-invasive urine collection using sodium polyacrylate-based diapers for PCR detection of uropathogens.Data in brief · 2024Article
50 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPCR inhibition by nucleic acid extracts is a well known yet poorly described phenomenon. Inhibition assessment generally depends on the assumption that inhibitors affect all PCR reactions to the same extent; i.e. that the reaction of interest and the control reaction are equally susceptible to inhibition. To test this assumption we performed inhibition assessment on DNA extracts from human urine samples, fresh urine and EDTA using different PCR reactions.
resultsWhen copurified inhibitors were assessed using two different PCR reactions one reaction appeared to be inhibited whilst the other was not. Further experiments using various concentrations of unextracted urine to inhibit six different PCR reactions revealed that susceptibility to inhibition was highly variable between reactions. Similar results were obtained using EDTA as the PCR inhibitor. We could find no obvious explanation why one reaction should be more susceptible to inhibition than another, although a possible association with amplicon GC content was noted.
conclusionThese findings have serious implications for all PCR-based gene expression studies, including the relatively new PCR array method, and for both qualitative and quantitative PCR-based molecular diagnostic assays, suggesting that careful consideration should be given to inhibition compatibility when conducting PCR analyses. We have demonstrated unequivocally that it is not safe to assume that different PCR reactions are equally susceptible to inhibition by substances co-purified in nucleic acid extracts.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.