ArticleJournal of cellular and molecular medicine2009
TRP-ML1 functions as a lysosomal NAADP-sensitive Ca2+ release channel in coronary arterial myocytes.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed, 91 citations in OpenAlex.
- HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors.Redox report : communications in free radical research · 2026Article
- HIV-1 gp120-induced lysosomal stress responses are controlled by TRPML1 redox sensors.bioRxiv : the preprint server for biology · 2026Article
- Lysosomal Ion Channels and Transporters: Recent Findings, Therapeutic Potential, and Technical Approaches.Bioelectricity · 2025Review
- CaBiomedicines · 2023Review
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Diversity of two-pore channels and the accessory NAADP receptors in intracellular CaCell calcium · 2022Review
- Regulation of exosome release by lysosomal acid ceramidase in coronary arterial endothelial cells: Role of TRPML1 channel.Current topics in membranes · 2022Article
- Lysosome function in glomerular health and disease.Cell and tissue research · 2021Review
- Vascular Dysfunction in Diabetes and Obesity: Focus on TRP Channels.Frontiers in physiology · 2021Review
- Lysosomal TRPML1 Channel: Implications in Cardiovascular and Kidney Diseases.Advances in experimental medicine and biology · 2021Article
- Roles of NADMolecules (Basel, Switzerland) · 2020Review
- Nanoparticle-Mediated Therapeutic Application for Modulation of Lysosomal Ion Channels and Functions.Pharmaceutics · 2020Review
- Arterial Medial Calcification through Enhanced small Extracellular Vesicle Release in Smooth Muscle-Specific Asah1 Gene Knockout Mice.Scientific reports · 2020Article
- Lysosomal size matters.Traffic (Copenhagen, Denmark) · 2020Review
- NAADP Receptors.Cold Spring Harbor perspectives in biology · 2019Review
- Control of lysosomal TRPML1 channel activity and exosome release by acid ceramidase in mouse podocytes.American journal of physiology. Cell physiology · 2019Article
- Patch-clamp technique to characterize ion channels in enlarged individual endolysosomes.Nature protocols · 2017Article
- Simvastatin promotes NPC1-mediated free cholesterol efflux from lysosomes through CYP7A1/LXRα signalling pathway in oxLDL-loaded macrophages.Journal of cellular and molecular medicine · 2017Article
- Disorders of lysosomal acidification-The emerging role of v-ATPase in aging and neurodegenerative disease.Ageing research reviews · 2016Review
- Lysosomal cholesterol accumulation in macrophages leading to coronary atherosclerosis in CD38(-/-) mice.Journal of cellular and molecular medicine · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent intracellular Ca(2+) signalling second messenger, but the mechanism of NAADP-induced Ca(2+) release is still poorly understood. The present study tested the hypothesis that NAADP induces Ca(2+) release from the lysosomal store via a TRP-ML1 (transient receptor potential-mucolipin 1)-mediated Ca(2+) release channel in coronary arterial myocytes (CAMs). RT-PCR and Western blot analyses demonstrated that TRP-ML1 was present in CAMs, and fluorescence resonance energy transfer (FRET) detection revealed that the TRP-ML1 was closely associated with some lysosomal proteins in these CAMs. ET-1, a well-known NAADP stimulator, was found to induce a local Ca(2+) burst from lysosomes followed by a global Ca(2+) release. This lysosome-associated Ca(2+) release was significantly inhibited in the TRP-ML1 siRNA pre-treated CAMs by 46.8 +/- 12.6% in the local Ca(2+) burst and 73.3 +/- 14.9% in the global Ca(2+) wave. In the reconstituted lysosomal channels from CAMs, NAADP activated Ca(2+) release channels at concentrations of 1-1000 nM, but neither activators (1 microM IP(3), 5 microM Rya) nor blockers (100 microM 2-APB, 50 microM Rya) of sarcoplasmic reticulum (SR) Ca(2+) release channels had effect on the channel activity. Moreover, TRP-ML1 gene silencing reduced this NAADP-sensitive Ca(2+) release channel activity in lysosomes by 71.5 +/- 18.5%. Immunoprecipitation or blockade of TRP-ML1 by anti-TRP-ML1 antibodies almost abolished NAADP-induced activation of lysosomal Ca(2+) channels (to 14.0 +/- 4.4% of control). These results for the first time provide direct evidence that an NAADP-sensitive Ca(2+) release channel is characteristic of TRP-ML1 channels.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.