ArticleJournal of clinical microbiology2008
Assay for 5' noncoding region analysis of all human rhinovirus prototype strains.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed.
- Comparison of asymptomatic and symptomatic rhinovirus infections in university students: incidence, species diversity, and viral load.Diagnostic microbiology and infectious disease · 2015Trial
- Molecular Types of Rhinovirus Among Cases of Acute Respiratory Infections in a University Hospital.Journal of medical virology · 2026Article
- Sensitive detection and differentiation of rhinoviruses and enteroviruses by a nested real-time RT-PCR assay.Microbiology spectrum · 2026Article
- Clinical Insights and Severity of Enterovirus D68 Respiratory Infections in Vietnamese Children.Open forum infectious diseases · 2026Article
- Full-length and near-full-length genomes of human rhinovirus A105 detected in patients with non-respiratory tract symptoms in Osaka, Japan.Microbiology resource announcements · 2025Article
- The added value of diagnostics to characterize age-specific patterns of respiratory viral infections and coinfections and to detect emerging threats.BMC infectious diseases · 2025Article
- Rhinovirus Genotypes Circulating in Bulgaria, 2018-2021.Viruses · 2023Article
- Etiologies of Acute Bronchiolitis in Children at Risk for Asthma, with Emphasis on the Human Rhinovirus Genotyping Protocol.Journal of clinical medicine · 2023Article
- Molecular characterization of coxsackievirus B5 from the sputum of pneumonia children patients of Kunming, Southwest China.Virology journal · 2023Article
- Comparison of Rhinovirus A-, B-, and C-Associated Respiratory Tract Illness Severity Based on the 5'-Untranslated Region Among Children Younger Than 5 Years.Open forum infectious diseases · 2022Article
- Molecular Epidemiology of Human Rhinovirus From 1-Year Surveillance Within a School Setting in Rural Coastal Kenya.Open forum infectious diseases · 2020Article
- A prospective case-control study on the association of Rhinovirus nasopharyngeal viral load and viremia in South African children hospitalized with severe pneumonia.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2020Article
- Molecular Subtyping of Human Rhinovirus in Children from Three Sub-Saharan African Countries.Journal of clinical microbiology · 2019Article
- The Emerging Role of Rhinoviruses in Lower Respiratory Tract Infections in Children - Clinical and Molecular Epidemiological Study From Croatia, 2017-2019.Frontiers in microbiology · 2019Article
- Epidemiological analysis and follow-up of human rhinovirus infection in children with asthma exacerbation.Journal of medical virology · 2018Article
- Rhinovirus C is associated with wheezing and rhinovirus A is associated with pneumonia in hospitalized children in Morocco.Journal of medical virology · 2017Article
- Phylogenetic analysis of human rhinoviruses collected over four successive years in Sydney, Australia.Influenza and other respiratory viruses · 2016Article
- Laboratory Diagnosis for Outbreak-Prone Infectious Diseases after Typhoon Yolanda (Haiyan), Philippines.PLoS currents · 2016Article
- Rhinovirus species and clinical features in children hospitalised with pneumonia from Mozambique.Tropical medicine & international health : TM & IH · 2016Article
- Newly Identified Enterovirus C Genotypes, Identified in the Netherlands through Routine Sequencing of All Enteroviruses Detected in Clinical Materials from 2008 to 2015.Journal of clinical microbiology · 2016Article
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Authors and funding
7 authors.
Funding
Abstract
Increasing recognition of the association of rhinovirus with severe lower respiratory tract illnesses has clarified the need to understand the relationship between specific serotypes of rhinovirus and their clinical consequences. To accomplish this, a specific and sensitive assay to detect and serotype rhinovirus directly from clinical specimens is needed. Traditional methods of serotyping using culture and serum neutralization are time-consuming, limited to certain reference laboratories, and complicated by the existence of over 100 serotypes of human rhinoviruses (HRVs). Accordingly, we have developed a sequence-based assay that targets a 390-bp fragment accounting for approximately two-thirds of the 5' noncoding region (NCR). Our goal was to develop an assay permitting amplification of target sequences directly from clinical specimens and distinction among all 101 prototype strains of rhinoviruses. We determined the sequences of all 101 prototype strains of HRV in this region to enable differentiation of virus genotypes in both viral isolates and clinical specimens. We evaluated this assay in a total of 101 clinical viral isolates and 24 clinical specimens and compared our findings to genotyping results using a different region of the HRV genome (the VP4-VP2 region). Five specimens associated with severe respiratory disease in children did not correlate with any known serotype of rhinovirus and were found to belong to a novel genogroup of rhinovirus, genogroup C. Isolates were also found that corresponded to the genogroup A2 variant identified in New York and Australia and two other novel group A clusters (GAC1 and GAC2).
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