ArticleJournal of clinical microbiology2008
Identification and evaluation of new target sequences for specific detection of Bordetella pertussis by real-time PCR.
Article in Journal of clinical microbiology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 53 citations in OpenAlex.
- A pangenome approach-based loop-mediated isothermal amplification assay for the specific and early detection of Bordetella pertussis.Scientific reports · 2023Article
- Article
- Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis.BMC microbiology · 2020Article
- A qPCR assay for Bordetella pertussis cells that enumerates both live and dead bacteria.PloS one · 2020Article
- Improving specificity of Bordetella pertussis detection using a four target real-time PCR.PloS one · 2017Article
- Comparing the Yield of Nasopharyngeal Swabs, Nasal Aspirates, and Induced Sputum for Detection of Bordetella pertussis in Hospitalized Infants.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2016Article
- Bordetella pertussis Strain Lacking Pertactin and Pertussis Toxin.Emerging infectious diseases · 2016Article
- Laboratory Diagnosis of Pertussis.Clinical microbiology reviews · 2015Review
- Isolation of High Level Macrolide Resistant Bordetella pertussis Without Transition Mutation at Domain V in Iran.Jundishapur journal of microbiology · 2015Article
- Survey and Rapid Detection of Bordetella pertussis in Clinical Samples Targeting the BP485 in China.Frontiers in public health · 2015Article
- Optimizing the Roche LightCycler(R) for Single-Tube Multiplexed RT-PCR Assays.Journal of clinical and diagnostic research : JCDR · 2015Article
- Status of pertussis in iran.Jundishapur journal of microbiology · 2014Article
- Evaluation of amplification targets for the specific detection of Bordetella pertussis using real-time polymerase chain reaction.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Multiplex real-time PCR assay for detection and differentiation of Bordetella pertussis and Bordetella parapertussis.Iranian journal of microbiology · 2014Article
- Mailed versus frozen transport of nasal swabs for surveillance of respiratory bacteria in remote Indigenous communities in Australia.BMC infectious diseases · 2013Article
- Comparison of culture and real-time PCR for detection of Bordetella pertussis isolated from patients in Iran.Iranian journal of microbiology · 2013Article
- Bead-based polymerase chain reaction on a microchip.Microfluidics and nanofluidics · 2012Article
- Performance of a novel microarray multiplex PCR for the detection of 23 respiratory pathogens (SYMP-ARI study).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Towards improved accuracy of Bordetella pertussis nucleic acid amplification tests.Journal of clinical microbiology · 2012Review
- Observational Research in Childhood Infectious Diseases (ORChID): a dynamic birth cohort study.BMJ open · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A comparative analysis of the Bordetella pertussis, B. bronchiseptica, and B. parapertussis genome assemblies permitted the identification of regions with significant sequence divergence and the design of two new real-time PCR assays, BP283 and BP485, for the specific detection of B. pertussis. The performance characteristics of these two assays were evaluated and compared to those of culture and an existing real-time PCR assay targeting the repetitive element IS481. The testing of 324 nasopharyngeal specimens indicated that, compared to culture, the BP283 assay had a sensitivity and specificity of 100 and 96.8% and the BP485 assay had a sensitivity and specificity of 92.3 and 97.1%. Notably, B. holmesii was isolated from two specimens that were positive by the IS481 assay but negative by the BP283 and BP485 assays. These two assays represent an improvement in specificity over those of PCR assays targeting only IS481 and may be duplexed or used in conjunction with existing PCR assays to improve the molecular detection of B. pertussis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.