ArticleJournal of the American Society of Nephrology : JASN2008
TLR4 facilitates translocation of bacteria across renal collecting duct cells.
Article in Journal of the American Society of Nephrology : JASN, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Obesity promotes urinary tract infection by disrupting bladder focal adhesion kinase signaling.iScience · 2025Article
- PTEN modulates urinary tract infection susceptibility and shapes urothelial antibacterial defenses.Life science alliance · 2025Article
- Uropathogen and host responses in pyelonephritis.Nature reviews. Nephrology · 2023Review
- Innate Bacteriostatic Mechanisms Defend the Urinary Tract.Annual review of physiology · 2022Review
- Expression and function of human ribonuclease 4 in the kidney and urinary tract.American journal of physiology. Renal physiology · 2021Article
- Innate immunity and urinary tract infection.Pediatric nephrology (Berlin, Germany) · 2020Review
- Podocyte Injury Through Interaction BetweenFrontiers in immunology · 2020Article
- Berberine ameliorates lipopolysaccharide‑induced inflammatory responses in mouse inner medullary collecting duct‑3 cells by downregulation of NF‑κB pathway.Molecular medicine reports · 2020Article
- Ribonuclease 7 Shields the Kidney and Bladder from Invasive UropathogenicJournal of the American Society of Nephrology : JASN · 2019Article
- Androgen exposure potentiates formation of intratubular communities and renal abscesses by Escherichia coli.Kidney international · 2018Article
- Cyclosporine A Induces MicroRNAs Controlling Innate Immunity during Renal Bacterial Infection.Journal of innate immunity · 2018Article
- High molecular weight hyaluronic acid: a two-pronged protectant against infection of the urogenital tract?Clinical & translational immunology · 2018Article
- β-Sitosterol and stigmasterol ameliorate dextran sulfate sodium-induced colitis in mice fed a high fat Western-style diet.Food & function · 2017Article
- Transcriptional Control of Dual Transporters Involved in α-Ketoglutarate Utilization Reveals Their Distinct Roles in UropathogenicFrontiers in microbiology · 2017Article
- Invasion of Host Cells and Tissues by Uropathogenic Bacteria.Microbiology spectrum · 2016Review
- Strengths and Limitations of Model Systems for the Study of Urinary Tract Infections and Related Pathologies.Microbiology and molecular biology reviews : MMBR · 2016Review
- Guggulsterone Attenuated Lipopolysaccharide-Induced Inflammatory Responses in Mouse Inner Medullary Collecting Duct-3 Cells.Inflammation · 2016Article
- A Murine Model for Escherichia coli Urinary Tract Infection.Methods in molecular biology (Clifton, N.J.) · 2016Article
- Amplifying renal immunity: the role of antimicrobial peptides in pyelonephritis.Nature reviews. Nephrology · 2015Review
- How the Innate Immune System Senses Trouble and Causes Trouble.Clinical journal of the American Society of Nephrology : CJASN · 2015Review
Corrections and comments
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Authors and funding
11 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Uropathogenic Escherichia coli (UPEC) are the most frequent causes of urinary tract infections and pyelonephritis. Renal medullary collecting duct (MCD) cells are the intrarenal site to which UPEC strains prefer to adhere and initiate an inflammatory response, but the ability of UPEC strains to translocate across impermeant MCD cells has not been demonstrated definitively. Here, several UPEC strains adhered to the apical surface and translocated across confluent murine inner MCD cells grown on filters. UPEC strains expressing cytolytic and vacuolating cytotoxins disrupted the integrity of cell layers, whereas noncytolytic UPEC strains passed through the cell layers without altering tight junctions. Apical-to-basal transcellular translocation was dramatically reduced after extinction of Toll-like receptor 4 (TLR4) and the lipid raft marker caveolin-1 by small interfering RNA. Furthermore, disruption of lipid raft integrity by filipin III and methyl-beta-cyclodextrin significantly reduced both the transcellular translocation of UPEC across murine inner MCD cell layers and the stimulation of proinflammatory mediators. Bacterial translocation was also significantly reduced in primary cultures of TLR4-deficient mouse MCD cells compared with MCD cells from wild-type mice. Benzyl alcohol, an anesthetic that enhances membrane fluidity, favored the recruitment of caveolin-1 in lipid rafts and increased the translocation of UPEC across cultured TLR4-deficient MCD cells. These findings demonstrate that the transcellular translocation of UPEC strains across impermeant layers of MCD cells may occur through lipid rafts via a TLR4-facilitated process.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.