ArticleNucleic acids research2008
A manganese-dependent ribozyme in the 3'-untranslated region of Xenopus Vg1 mRNA.
Article in Nucleic acids research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- RNA sample optimization for cryo-EM analysis.Nature protocols · 2025Review
- Sequence-Dependent Acylation of Peptide Lysine Residues by DNAzymes.Chembiochem : a European journal of chemical biology · 2024Article
- Development and validation of a generic methyltransferase enzymatic assay based on an SAH riboswitch.SLAS discovery : advancing life sciences R & D · 2024Article
- A comparative survey of the influence of small self-cleaving ribozymes on gene expression in human cell culture.RNA biology · 2024Article
- Ribocentre: a database of ribozymes.Nucleic acids research · 2023Article
- Single-round deoxyribozyme discovery.Nucleic acids research · 2021Article
- Hovlinc is a recently evolved class of ribozyme found in human lncRNA.Nature chemical biology · 2021Article
- Prebiological Membranes and Their Role in the Emergence of Early Cellular Life.The Journal of membrane biology · 2020Review
- The 3'-untranslated region of mRNAs as a site for ribozyme cleavage-dependent processing and control in bacteria.RNA biology · 2017Article
- Improved deoxyribozymes for synthesis of covalently branched DNA and RNA.Nucleic acids research · 2011Article
- On the origin of life in the zinc world. 2. Validation of the hypothesis on the photosynthesizing zinc sulfide edifices as cradles of life on Earth.Biology direct · 2009Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The smallest catalytic RNA identified to date is a manganese-dependent ribozyme that requires only a complex between GAAA and UUU to effect site-specific cleavage. We show here that this ribozyme occurs naturally in the 3'-UTR of Vg1 and beta-actin mRNAs. In accord with earlier studies with model RNAs, cleavage occurs only in the presence of manganese or cadmium ions and proceeds optimally near 30 degrees C and physiological pH. The time course of cleavage in Vg1 mRNA best fits a two-step process in which both steps are first-order. In Vg1 mRNA, the ribozyme is positioned adjacent to a polyadenylation signal, but has no influence on translation of the mRNA in Xenopus oocytes. Putative GAAA ribozyme structures are also near polyadenylation sites in yeast and rat actin mRNAs. Analysis of sequences in the PolyA Cleavage Site and 3'-UTR Database (PACdb) revealed no particular bias in the frequency or distribution of the GAAA motif that would suggest that this ribozyme is currently or was recently used for cleavage to generate processed transcripts. Nonetheless, we speculate that the complementary strands that comprise the ribozyme may account for the origin of sequence elements that direct present-day 3'-end processing of eukaryotic mRNAs.
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