ArticleJournal of cellular and molecular medicine2009
CD34+ cells augment endothelial cell differentiation of CD14+ endothelial progenitor cells in vitro.
Article in Journal of cellular and molecular medicine, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 47 citations in OpenAlex.
- Resident vascular Sca1iScience · 2024Article
- Stem Cell Therapy in Limb Ischemia: State-of-Art, Perspective, and Possible Impacts of Endometrial-Derived Stem Cells.Frontiers in cell and developmental biology · 2022Review
- Flow-through isolation of human first trimester umbilical cord endothelial cells.Histochemistry and cell biology · 2021Article
- The Effect of Sex Differences on Endothelial Function and Circulating Endothelial Progenitor Cells in Hypertriglyceridemia.Cardiology research and practice · 2020Article
- DKK3 (Dikkopf-3) Transdifferentiates Fibroblasts Into Functional Endothelial Cells-Brief Report.Arteriosclerosis, thrombosis, and vascular biology · 2019Article
- Endothelial Progenitor Cells for the Vascularization of Engineered Tissues.Tissue engineering. Part B, Reviews · 2018Review
- From top to bottom: midkine and pleiotrophin as emerging players in immune regulation.Journal of leukocyte biology · 2017Review
- Concise Review: Endothelial Progenitor Cells in Regenerative Medicine: Applications and Challenges.Stem cells translational medicine · 2016Review
- Early outgrowth cells versus endothelial colony forming cells functions in platelet aggregation.Journal of translational medicine · 2015Article
- Myocardial Ischemic Subject's Thymus Fat: A Novel Source of Multipotent Stromal Cells.PloS one · 2015Article
- Occurring of In Vitro Functional Vasculogenic Pericytes from Human Circulating Early Endothelial Precursor Cell Culture.Stem cells international · 2015Article
- Article
- Interactions between endothelial progenitor cells (EPC) and titanium implant surfaces.Clinical oral investigations · 2013Article
- Endothelial progenitor cells in atherosclerosis.Frontiers in bioscience (Landmark edition) · 2012Review
- Chronic Type A aortic dissection: could surgical intervention be guided by molecular markers?Journal of cellular and molecular medicine · 2011Article
- Improved culture-based isolation of differentiating endothelial progenitor cells from mouse bone marrow mononuclear cells.PloS one · 2011Article
- Endothelial progenitor cell transplantation improves long-term stroke outcome in mice.Annals of neurology · 2010Article
- The promise of cell-based therapies for diabetic complications: challenges and solutions.Circulation research · 2010Review
- Endothelial progenitor cell dysfunction in patients with progressive chronic kidney disease.American journal of physiology. Renal physiology · 2009 · on this mapArticle
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Neovascularization by endothelial progenitor cells (EPC) for the treatment of ischaemic diseases has been a topic of intense research. The CD34(+) cell is often designated as EPC, because it contributes to repair of ischaemic injuries through neovascularization. However, incorporation of CD34(+) cells into the neovasculature is limited, suggesting another role which could be paracrine. CD14(+) cells can also differentiate into endothelial cells and contribute to neovascularization. However, the low proliferative capacity of CD14(+) cell-derived endothelial cells hampers their use as therapeutic cells. We made the assumption that an interaction between CD34(+) and CD14(+) cells augments endothelial differentiation of the CD14(+) cells. In vitro, the influence of CD34(+) cells on the endothelial differentiation capacity of CD14(+) cells was investigated. Endothelial differentiation was analysed by expression of endothelial cell markers CD31, CD144, von Willebrand Factor and endothelial Nitric Oxide Synthase. Furthermore, we assessed proliferative capacity and endothelial cell function of the cells in culture. In monocultures, 63% of the CD14(+)-derived cells adopted an endothelial cell phenotype, whereas in CD34(+)/CD14(+) co-cultures 95% of the cells showed endothelial cell differentiation. Proliferation increased up to 12% in the CD34(+)/CD14(+) co-cultures compared to both monocultures. CD34-conditioned medium also increased endothelial differentiation of CD14(+) cells. This effect was abrogated by hepatocyte growth factor neutralizing antibodies, but not by interleukin-8 and monocyte chemoattractant protein-1 neutralizing antibodies. We show that co-culturing of CD34(+) and CD14(+) cells results in a proliferating population of functional endothelial cells, which may be suitable for treatment of ischaemic diseases such as myocardial infarction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.