ArticlePLoS biology2008
Dual role of topoisomerase II in centromere resolution and aurora B activity.
Article in PLoS biology, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 76 citations in OpenAlex.
- Safeguarding genome integrity: Polo-like kinase Cdc5 and phosphatase Cdc14 orchestrate Topoisomerase II-mediated catenane resolution in mitosis.Nucleic acids research · 2026Article
- High-throughput screening identifies Aurora kinase B as a critical therapeutic target for Merkel cell carcinoma.Nature communications · 2025Article
- Defining a core configuration for human centromeres during mitosis.Nature communications · 2023Article
- Defining a core configuration for human centromeres during mitosis.bioRxiv : the preprint server for biology · 2023Article
- Super-resolution microscopy reveals the number and distribution of topoisomerase IIα and CENH3 molecules within barley metaphase chromosomes.Chromosoma · 2023Article
- Anaphase B: Long-standing models meet new concepts.Seminars in cell & developmental biology · 2021Review
- The noncanonical role of the protease cathepsin D as a cofilin phosphatase.Cell research · 2021Article
- Aurora B and C kinases regulate chromosome desynapsis and segregation during mouse and human spermatogenesis.Journal of cell science · 2020Article
- Review
- Topoisomerase IIα is essential for maintenance of mitotic chromosome structure.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Topoisomerase II SUMOylation activates a metaphase checkpoint via Haspin and Aurora B kinases.The Journal of cell biology · 2020Article
- Phenotypic characterization of diamond (dind), a Drosophila gene required for multiple aspects of cell division.Chromosoma · 2018Article
- 3-O-acetyl-11-keto-β-boswellic acid exerts anti-tumor effects in glioblastoma by arresting cell cycle at G2/M phase.Journal of experimental & clinical cancer research : CR · 2018Article
- A Topology-Centric View on Mitotic Chromosome Architecture.International journal of molecular sciences · 2017Review
- The Hybrid Incompatibility GenesGenetics · 2017Article
- YM155 inhibits topoisomerase function.Anti-cancer drugs · 2017Article
- Natural Compounds as Anticancer Agents Targeting DNA Topoisomerases.Current genomics · 2017Review
- The Ins and Outs of Aurora B Inner Centromere Localization.Frontiers in cell and developmental biology · 2017Review
- Naa50/San-dependent N-terminal acetylation of Scc1 is potentially important for sister chromatid cohesion.Scientific reports · 2016Article
- RNF168 and USP10 regulate topoisomerase IIα function via opposing effects on its ubiquitylation.Nature communications · 2016Article
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6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromosome segregation requires sister chromatid resolution. Condensins are essential for this process since they organize an axial structure where topoisomerase II can work. How sister chromatid separation is coordinated with chromosome condensation and decatenation activity remains unknown. We combined four-dimensional (4D) microscopy, RNA interference (RNAi), and biochemical analyses to show that topoisomerase II plays an essential role in this process. Either depletion of topoisomerase II or exposure to specific anti-topoisomerase II inhibitors causes centromere nondisjunction, associated with syntelic chromosome attachments. However, cells degrade cohesins and timely exit mitosis after satisfying the spindle assembly checkpoint. Moreover, in topoisomerase II-depleted cells, Aurora B and INCENP fail to transfer to the central spindle in late mitosis and remain tightly associated with centromeres of nondisjoined sister chromatids. Also, in topoisomerase II-depleted cells, Aurora B shows significantly reduced kinase activity both in S2 and HeLa cells. Codepletion of BubR1 in S2 cells restores Aurora B kinase activity, and consequently, most syntelic attachments are released. Taken together, our results support that topoisomerase II ensures proper sister chromatid separation through a direct role in centromere resolution and prevents incorrect microtubule-kinetochore attachments by allowing proper activation of Aurora B kinase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.