ArticleThe Journal of cell biology1991
Microinjection of the catalytic fragment of myosin light chain kinase into dividing cells: effects on mitosis and cytokinesis.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
35 citing papers in PubMed, 96 citations in OpenAlex.
- Heterologously secreted MbxA from Moraxella bovis induces a membrane blebbing response of the human host cell.Scientific reports · 2022Article
- Amoeboid-like migration ensures correct horizontal cell layer formation in the developing vertebrate retina.eLife · 2022Article
- Myosin light chain kinase-driven myosin II turnover regulates actin cortex contractility during mitosis.Molecular biology of the cell · 2021Article
- Article
- Focal adhesion kinase is activated by microtubule-depolymerizing agents and regulates membrane blebbing in human endothelial cells.Journal of cellular and molecular medicine · 2020Article
- Dynamic blebbing: A bottleneck to human embryonic stem cell culture that can be overcome by Laminin-Integrin signaling.Stem cell research · 2018Article
- Differential role of nonmuscle myosin II isoforms during blebbing of MCF-7 cells.Molecular biology of the cell · 2017Article
- Drak Is Required for Actomyosin Organization During Drosophila Cellularization.G3 (Bethesda, Md.) · 2016Article
- Cytokinesis involves a nontranscriptional function of the Hippo pathway effector YAP.Science signaling · 2016Article
- Involvement of Membrane Blebbing in Immunological Disorders and Cancer.Medical principles and practice : international journal of the Kuwait University, Health Science Centre · 2016Review
- An equatorial contractile mechanism drives cell elongation but not cell division.PLoS biology · 2014Article
- A computational model of bleb formation.Mathematical medicine and biology : a journal of the IMA · 2013Article
- Determination of the lowest concentrations of aldehyde fixatives for completely fixing various cellular structures by real-time imaging and quantification.Histochemistry and cell biology · 2013Article
- Article
- Molecular networks linked by Moesin drive remodeling of the cell cortex during mitosis.The Journal of cell biology · 2011Article
- A transcribed pseudogene of MYLK promotes cell proliferation.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Mitosis: spindle evolution and the matrix model.Protoplasma · 2009Article
- Mechanical and biochemical modeling of cortical oscillations in spreading cells.Biophysical journal · 2008Article
- Implications of a poroelastic cytoplasm for the dynamics of animal cell shape.Seminars in cell & developmental biology · 2008Review
- Life and times of a cellular bleb.Biophysical journal · 2008Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Myosin light chain kinase (MLCK) is thought to regulate the contractile activity in smooth and non-muscle cells, and may play an important role in controlling the reorganization of the actin-myosin cytoskeleton during cell division. To test this hypothesis we have microinjected the 61-kD catalytic fragment of MLCK into mitotic cells, and examined the effects of unregulated MLCK activity on cell division. The microinjection of active 61 kD causes both a significant delay in the transit time from nuclear envelope breakdown to anaphase onset, and an increase in motile surface activity during and after metaphase. Control experiments with intact MLCK or with inactive catalytic fragment suggest that these effects are specifically induced by the unregulated myosin light chain kinase activity. Immunofluorescence analysis suggests that delays in mitosis are coupled to disruptions of spindle structures, while increased surface motility may be related to changes in the organization of actin and myosin at the cell cortex. Most importantly, despite the expression of strong phenotypes, 61 kD-injected cells still form functional cleavage furrows that progress through cytokinesis at rates identical to those of control cells. Together, these results suggest that the activity of MLCK can affect mitosis and cortical activities, however additional control mechanisms are likely involved in the regulation of cytokinesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.