ArticleThe Journal of cell biology1991
Organization of the sea urchin egg endoplasmic reticulum and its reorganization at fertilization.
Article in The Journal of cell biology, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
64 citing papers in PubMed, 224 citations in OpenAlex.
- Membrane Protein Folding and Biogenesis: Insights from Single-Molecule Force Spectroscopy.Chemical reviews · 2026Review
- Endoplasmic reticulum disruption stimulates nuclear membrane mechanotransduction.Nature cell biology · 2026Article
- Mitotic spindle membranes.Molecular biology of the cell · 2025Review
- Solubility phase transition of maternal RNAs during vertebrate oocyte-to-embryo transition.Developmental cell · 2023Article
- Regulation of RNA localization during oocyte maturation by dynamic RNA-ER association and remodeling of the ER.Cell reports · 2022Article
- Changes in cortical endoplasmic reticulum clusters in the fertilized mouse oocyte†.Biology of reproduction · 2022Article
- Dynamic changes in the association between maternal mRNAs and endoplasmic reticulum during ascidian early embryogenesis.Development genes and evolution · 2022Article
- An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization.PloS one · 2022Article
- Article
- Embryo, larval, and juvenile staging of Lytechinus pictus from fertilization through sexual maturation.Developmental dynamics : an official publication of the American Association of Anatomists · 2020Article
- The Perinuclear ER Scales Nuclear Size Independently of Cell Size in Early Embryos.Developmental cell · 2020Article
- Review
- ER morphology and endo-lysosomal crosstalk: Functions and disease implications.Biochimica et biophysica acta. Molecular and cell biology of lipids · 2020Review
- Review
- Cytoskeleton Force Exertion in Bulk Cytoplasm.Frontiers in cell and developmental biology · 2020Review
- A centrosome-localized calcium signal is essential for mammalian cell mitosis.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation.Journal of lipid research · 2019Article
- Asymmetric division through a reduction of microtubule centering forces.The Journal of cell biology · 2019Article
- Physical Forces Determining the Persistency and Centering Precision of Microtubule Asters.Nature physics · 2018Article
- Advances in Plant ER Architecture and Dynamics.Plant physiology · 2018Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The ER of eggs of the sea urchin Lytechinus pictus was stained by microinjecting a saturated solution of the fluorescent dicarbocyanine DiIC18(3) (DiI) in soybean oil; the dye spread from the oil drop into ER membranes throughout the egg but not into other organelles. Confocal microscopy revealed large cisternae extending throughout the interior of the egg and a tubular membrane network at the cortex. Since diffusion of DiI is confined to continuous bilayers, the spread of the dye supports the concept that the ER is a cell-wide, interconnected compartment. In time lapse observations, the internal cisternae were seen to be in continuous motion, while the cortical ER was stationary. After fertilization, the internal ER appeared to become more finely divided, beginning as a wave apparently coincident with the calcium wave and becoming most marked by 2-3 min. By 5-8 min the ER returned to an organization similar to that of the unfertilized egg. The cortical network also changed at fertilization; it became disrupted and eventually recovered. DiI labeling allowed continuous observations of the ER during pronuclear migration and mitosis. DiI-stained membranes accumulated in the region of the microtubule array surrounding the sperm nucleus and centriole (the sperm aster) as it migrated to the center of the egg; this accumulation persisted near the centrosomes and zygote nucleus throughout pronuclear fusion and the first two mitotic cycles. We have used a new method to observe the spatial and temporal organization of the ER in a living cell, and we have demonstrated a striking reorganization of the ER at fertilization.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.