ArticleJournal of proteome research2008
Membrane glycoproteins associated with breast tumor cell progression identified by a lectin affinity approach.
Article in Journal of proteome research, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 69 citations in OpenAlex.
- MALDI Matrix: Origins, Innovations, and Frontiers.Chemical reviews · 2026Review
- Optimization of glycopeptide enrichment techniques for the identification of clinical biomarkers.Expert review of proteomics · 2024Review
- David M. Lubman-The University of Michigan-A retrospective in research.Mass spectrometry reviews · 2023Review
- A Pragmatic Guide to Enrichment Strategies for Mass Spectrometry-Based Glycoproteomics.Molecular & cellular proteomics : MCP · 2021Review
- Glycoproteomic markers of hepatocellular carcinoma-mass spectrometry based approaches.Mass spectrometry reviews · 2019Review
- Plasma Glycoproteomic Study of Therapeutic Hypothermia Reveals Novel Markers Predicting Neurologic Outcome Post-cardiac Arrest.Translational stroke research · 2018Article
- Comparison of Syndecan-1 Immunohistochemical Expression in Lobular and Ductal Breast Carcinoma with Nodal Metastases.Analytical cellular pathology (Amsterdam) · 2018Article
- Glycoprotein Enrichment Analytical Techniques: Advantages and Disadvantages.Methods in enzymology · 2017Article
- Real-time and label-free analysis of binding thermodynamics of carbohydrate-protein interactions on unfixed cancer cell surfaces using a QCM biosensor.Scientific reports · 2015Article
- Galectin-3 Binding Protein Secreted by Breast Cancer Cells Inhibits Monocyte-Derived Fibrocyte Differentiation.Journal of immunology (Baltimore, Md. : 1950) · 2015Article
- Defining glycoprotein cancer biomarkers by MS in conjunction with glycoprotein enrichment.Biomarkers in medicine · 2015Review
- LC-MS/MS quantitation of esophagus disease blood serum glycoproteins by enrichment with hydrazide chemistry and lectin affinity chromatography.Journal of proteome research · 2014Article
- GRP78 is a novel receptor initiating a vascular barrier protective response to oxidized phospholipids.Molecular biology of the cell · 2014Article
- Using a cell line breast cancer progression system to identify biomarker candidates.Journal of proteomics · 2014Article
- Complement c5a generation by staphylococcal biofilms.Shock (Augusta, Ga.) · 2013Article
- High-throughput screening of tumor metastatic-related differential glycoprotein in hepatocellular carcinoma by iTRAQ combines lectin-related techniques.Medical oncology (Northwood, London, England) · 2013Article
- Lectin chromatography/mass spectrometry discovery workflow identifies putative biomarkers of aggressive breast cancers.Journal of proteome research · 2012Article
- Identification of glycoprotein markers for pancreatic cancer CD24+CD44+ stem-like cells using nano-LC-MS/MS and tissue microarray.Journal of proteome research · 2012Article
- Article
- Metastasis-associated cell surface oncoproteomics.Frontiers in pharmacology · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
The membrane glycoprotein component of the cellular proteome represents a promising source for potential disease biomarkers and therapeutic targets. Here we describe the development of a method that facilitates the analysis of membrane glycoproteins and apply it to the differential analysis of breast tumor cells with distinct malignant phenotypes. The approach combines two membrane extraction procedures, and enrichment using ConA and WGA lectin affinity columns, prior to digestion and analysis by LC-MS/MS. The glycoproteins are identified and quantified by spectral counting. Although the distribution of glycoprotein expression as a function of MW and p I was very similar between the two related cell lines tested, the approach enabled the identification of several distinct membrane glycoproteins with an expression index correlated with either a precancerous (MCF10AT1), or a malignant, metastatic cellular phenotype (MCF10CA1a). Among the proteins associated with the malignant phenotype, Gamma-glutamyl hydrolase, CD44, Galectin-3-binding protein, and Syndecan-1 protein have been reported as potential biomarkers of breast cancer.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.