ArticleBioconjugate chemistry2008
Amphiphilic block copolymers enhance cellular uptake and nuclear entry of polyplex-delivered DNA.
Article in Bioconjugate chemistry, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The trial behind it
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Who cites it
34 citing papers in PubMed, 96 citations in OpenAlex.
- Eradication of cancer stem cells in triple negative breast cancer using doxorubicin/pluronic polymeric micelles.Nanomedicine : nanotechnology, biology, and medicine · 2020Article
- AKT2 siRNA delivery with amphiphilic-based polymeric micelles show efficacy against cancer stem cells.Drug delivery · 2018Article
- Engineering T7 bacteriophage as a potential DNA vaccine targeting delivery vector.Virology journal · 2018Article
- Transfection of bovine fetal fibroblast with polyethylenimine (PEI) nanoparticles: effect of particle size and presence of fetal bovine serum on transgene delivery and cytotoxicity.Cytotechnology · 2017Article
- Poloxamine/fibrin hybrid hydrogels for non-viral gene delivery.Journal of tissue engineering and regenerative medicine · 2017Article
- Horizontal gene transfer from macrophages to ischemic muscles upon delivery of naked DNA with Pluronic block copolymers.Biomaterials · 2016Article
- Phytantriol Based "Stealth" Lyotropic Liquid Crystalline Nanoparticles for Improved Antitumor Efficacy and Reduced Toxicity of Docetaxel.Pharmaceutical research · 2015Article
- A Low Protein Binding Cationic Poly(2-oxazoline) as Non-Viral Vector.Macromolecular bioscience · 2015Article
- Amphiphilic macromolecules on cell membranes: from protective layers to controlled permeabilization.The Journal of membrane biology · 2014Review
- SERCA2a gene therapy can improve symptomatic heart failure in δ-sarcoglycan-deficient animals.Human gene therapy · 2014Article
- Recent advances in delivery of drug-nucleic acid combinations for cancer treatment.Journal of controlled release : official journal of the Controlled Release Society · 2013Review
- Polyplex-induced cytosolic nuclease activation leads to differential transgene expression.Molecular pharmaceutics · 2013Article
- The membrane-active tri-block copolymer pluronic F-68 profoundly rescues rat hippocampal neurons from oxygen-glucose deprivation-induced death through early inhibition of apoptosis.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2013Article
- Bioreducible polyether-based pDNA ternary polyplexes: balancing particle stability and transfection efficiency.Colloids and surfaces. B, Biointerfaces · 2012Article
- Exploring the mechanism of plasmid DNA nuclear internalization with polymer-based vehicles.Molecular pharmaceutics · 2012Article
- Poly(alkylene oxide) copolymers for nucleic acid delivery.Accounts of chemical research · 2012Article
- Self-assembled supramolecular nano vesicles for safe and highly efficient gene delivery to solid tumors.International journal of nanomedicine · 2012Article
- Degradable gene delivery systems based on Pluronics-modified low-molecular-weight polyethylenimine: preparation, characterization, intracellular trafficking, and cellular distribution.International journal of nanomedicine · 2012Article
- Amiloride enhances antigen specific CTL by faciliting HBV DNA vaccine entry into cells.PloS one · 2012Article
- Combined pluronic P85- and ultrasound contrast agents-mediated gene transfection to HepG2 cells.Journal of Huazhong University of Science and Technology. Medical sciences = Hua zhong ke ji da xue xue bao. Yi xue Ying De wen ban = Huazhong keji daxue xuebao. Yixue Yingdewen ban · 2011Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
This work for the first time demonstrates that synthetic polymers enhance uptake and nuclear import of plasmid DNA (pDNA) through the activation of cellular trafficking machinery. Nonionic block copolymers of poly(ethylene oxide) and poly(propylene oxide), Pluronics, are widely used as excipients in pharmaceutics. We previously demonstrated that Pluronics increase the phosphorylation of IkappaB and subsequent NFkappaB nuclear localization as well as upregulate numerous NFkappaB-related genes. In this study, we show that Pluronics enhance gene transfer by pDNA/polycation complexes ("polyplexes") in a promoter-dependent fashion. Addition of Pluronic P123 or P85 to polyethyleneimine-based polyplexes had little effect on polyplex particle size but significantly enhanced pDNA cellular uptake, nuclear translocation, and gene expression in several cell lines. When added to polyplex-transfected cells after transfection, Pluronics enhanced nuclear import of pDNA containing NFkappaB binding sites, but have no effect on import of pDNA without these sites. Altogether, our studies suggest that Pluronics rapidly activate NFkappaB, which binds cytosolic pDNA that possesses promoters containing NFkappaB binding sites and consequently increase nuclear import of pDNA through NFkappaB nuclear translocation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.