ArticleMolecular vision2008
Cyan fluorescent protein expression in ganglion and amacrine cells in a thy1-CFP transgenic mouse retina.
Article in Molecular vision, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 55 papers.
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Who cites it
55 citing papers in PubMed, 84 citations in OpenAlex.
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- Comprehensive single-cell atlas of the mouse retina.iScience · 2024Article
- Optimizing retinal ganglion cell nuclear staining for automated cell counting.Experimental eye research · 2024Article
- Senolytic and senomorphic agent procyanidin C1 alleviates structural and functional decline in the aged retina.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Comprehensive single-cell atlas of the mouse retina.bioRxiv : the preprint server for biology · 2024Article
- A novel GCaMP6f-RCS rat model for studying electrical stimulation in the degenerated retina.Frontiers in cell and developmental biology · 2024Article
- Pan-retinal ganglion cell markers in mice, rats, and rhesus macaques.Zoological research · 2023Article
- Elk-1 regulates retinal ganglion cell axon regeneration after injury.Scientific reports · 2022Article
- Implications of Neural Plasticity in Retinal Prosthesis.Investigative ophthalmology & visual science · 2022Article
- Label-free highly multimodal nonlinear endoscope.Optics express · 2022Article
- Tools and Biomarkers for the Study of Retinal Ganglion Cell Degeneration.International journal of molecular sciences · 2022Review
- Light-Sheet Microscopy of the Optic Nerve Reveals Axonal Degeneration and Microglial Activation in NMDA-Induced Retinal Injury.EC ophthalmology · 2021Article
- A Tropomycin-Related Kinase B Receptor Activator for the Management of Ocular Blast-Induced Vision Loss.Journal of neurotrauma · 2021Article
- Mutant three-repeat tau expression initiates retinal ganglion cell death through Caspase-2.Neurobiology of disease · 2021Article
- Transgenic models for investigating the nervous system: Currently available neurofluorescent reporters and potential neuronal markers.Biochimica et biophysica acta. General subjects · 2020Review
- RBFOX3/NeuN is dispensable for visual function.PloS one · 2018Article
- Polymodal TRPV1 and TRPV4 Sensors Colocalize but Do Not Functionally Interact in a Subpopulation of Mouse Retinal Ganglion Cells.Frontiers in cellular neuroscience · 2018Article
- Assessing retinal ganglion cell damage.Eye (London, England) · 2017Review
- Evaluating retinal ganglion cell loss and dysfunction.Experimental eye research · 2016Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
purposeTo characterize cyan fluorescent protein (CFP) expression in the retina of the thy1-CFP (B6.Cg-Tg(Thy1-CFP)23Jrs/J) transgenic mouse line.
methodsCFP expression was characterized using morphometric methods and immunohistochemistry with antibodies to neurofilament light (NF-L), neuronal nuclei (NeuN), POU-domain protein (Brn3a) and calretinin, which immunolabel ganglion cells, and syntaxin 1 (HPC-1), glutamate decarboxylase 67 (GAD(67)), GABA plasma membrane transporter-1 (GAT-1), and choline acetyltransferase (ChAT), which immunolabel amacrine cells.
resultsCFP was extensively expressed in the inner retina, primarily in the inner plexiform layer (IPL), ganglion cell layer (GCL), nerve fiber layer, and optic nerve. CFP fluorescent cell bodies were in all retinal regions and their processes ramified in all laminae of the IPL. Some small, weakly CFP fluorescent somata were in the inner nuclear layer (INL). CFP-containing somata in the GCL ranged from 6 to 20 microm in diameter, and they had a density of 2636+/-347 cells/mm2 at 1.5 mm from the optic nerve head. Immunohistochemical studies demonstrated colocalization of CFP with the ganglion cell markers NF-L, NeuN, Brn3a, and calretinin. Immunohistochemistry with antibodies to HPC-1, GAD(67), GAT-1, and ChAT indicated that the small, weakly fluorescent CFP cells in the INL and GCL were cholinergic amacrine cells.
conclusionsThe total number and density of CFP-fluorescent cells in the GCL were within the range of previous estimates of the total number of ganglion cells in the C57BL/6J line. Together these findings suggest that most ganglion cells in the thy1-CFP mouse line 23 express CFP. In conclusion, the thy1-CFP mouse line is highly useful for studies requiring the identification of ganglion cells.
Indexed as
Identifiers
18728756PMC2519030W1480449452What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.