ArticleProceedings of the National Academy of Sciences of the United States of America2008
Genetically encoded photoswitching of actin assembly through the Cdc42-WASP-Arp2/3 complex pathway.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
56 citing papers in PubMed, 103 citations in OpenAlex.
- Two Decades of Optogenetic Tools: A Retrospective and a Look Ahead.Advanced genetics (Hoboken, N.J.) · 2025Review
- A Chemogenetic Toolkit for Inducible, Cell Type-Specific Actin Disassembly.Small methods · 2025Article
- PI(3,4,5)P3-mediated Cdc42 activation regulates macrophage podosome assembly.Cellular and molecular life sciences : CMLS · 2025Article
- Extracellular Optogenetics to Interrogate Unmodified Primary Cell Functions.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Direct investigation of cell contraction signal networks by light-based perturbation methods.Pflugers Archiv : European journal of physiology · 2023Review
- Light-inducible T cell engagers trigger, tune, and shape the activation of primary T cells.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- miR-34a induces neutrophil apoptosis by regulating Cdc42-WASP-Arp2/3 pathway-mediated F-actin remodeling and ROS production.Redox report : communications in free radical research · 2022Article
- Next Generation Opto-Jasplakinolides Enable Local Remodeling of Actin Networks.Angewandte Chemie (International ed. in English) · 2022Article
- The Red Edge: Bilin-Binding Photoreceptors as Optogenetic Tools and Fluorescence Reporters.Chemical reviews · 2021Review
- Optogenetic approaches for understanding homeostatic and degenerative processes in Drosophila.Cellular and molecular life sciences : CMLS · 2021Review
- A light way for nuclear cell biologists.Journal of biochemistry · 2021Review
- Optogenetics: The Art of Illuminating Complex Signaling Pathways.Physiology (Bethesda, Md.) · 2021Review
- A far-red cyanobacteriochrome lineage specific for verdins.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Production, Purification and Characterization of Recombinant Biotinylated Phytochrome B for Extracellular Optogenetics.Bio-protocol · 2020Article
- The effects of the M2a macrophage-induced axonal regeneration of neurons by arginase 1.Bioscience reports · 2020Article
- A Computational Protocol for Regulating Protein Binding Reactions with a Light-Sensitive Protein Dimer.Journal of molecular biology · 2020Article
- From membrane receptors to protein synthesis and actin cytoskeleton: Mechanisms underlying long lasting forms of synaptic plasticity.Seminars in cell & developmental biology · 2019Review
- Photodimerization systems for regulating protein-protein interactions with light.Current opinion in structural biology · 2019Review
- Review
- A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs.Nature communications · 2017Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
Abstract
General methods to engineer genetically encoded, reversible, light-mediated control over protein function would be useful in many areas of biomedical research and technology. We describe a system that yields such photo-control over actin assembly. We fused the Rho family GTPase Cdc42 in its GDP-bound form to the photosensory domain of phytochrome B (PhyB) and fused the Cdc42 effector, the Wiskott-Aldrich Syndrome Protein (WASP), to the light-dependent PhyB-binding domain of phytochrome interacting factor 3 (Pif3). Upon red light illumination, the fusion proteins bind each other, activating WASP, and consequently stimulating actin assembly by the WASP target, the Arp2/3 complex. Binding and WASP activation are reversed by far-red illumination. Our approach, in which the biochemical specificity of the nucleotide switch in Cdc42 is overridden by the light-dependent PhyB-Pif3 interaction, should be generally applicable to other GTPase-effector pairs.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.