ArticleJournal of cellular physiology2009
Thrombospondin-1-induced apoptosis of brain microvascular endothelial cells can be mediated by TNF-R1.
Article in Journal of cellular physiology, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Regulated programmed cell death in acute lung injury: from pathogenesis to therapy.Frontiers in immunology · 2025Review
- Plasma THBS1 as a predictive biomarker for poor prognosis and brain metastasis in patients with HER2-enriched breast cancer.International journal of clinical oncology · 2024Article
- PP2A Affects Angiogenesis via Its Interaction with a Novel Phosphorylation Site of TSP1.International journal of molecular sciences · 2024Article
- Isthmin-A Multifaceted Protein Family.Cells · 2022Review
- Functions of Thrombospondin-1 in the Tumor Microenvironment.International journal of molecular sciences · 2021Review
- miR-195 reduces age-related blood-brain barrier leakage caused by thrombospondin-1-mediated selective autophagy.Aging cell · 2020Article
- Quantitative Proteomics Reveals the Beneficial Effects of Low Glucose on Neuronal Cell Survival in anFrontiers in cellular neuroscience · 2020Article
- CD36 inhibits β-catenin/c-myc-mediated glycolysis through ubiquitination of GPC4 to repress colorectal tumorigenesis.Nature communications · 2019Article
- Article
- Tetrahydrobiopterin (BH4) deficiency is associated with augmented inflammation and microvascular degeneration in the retina.Journal of neuroinflammation · 2017Article
- The Roles of Thrombospondins in Hemorrhagic Stroke.BioMed research international · 2017Review
- The Role of Clinical Proteomics, Lipidomics, and Genomics in the Diagnosis of Alzheimer's Disease.Proteomes · 2016Article
- Extracellular Vesicles Activate a CD36-Dependent Signaling Pathway to Inhibit Microvascular Endothelial Cell Migration and Tube Formation.Arteriosclerosis, thrombosis, and vascular biology · 2016Article
- Regulatory mechanisms of testosterone-stimulated song in the sensorimotor nucleus HVC of female songbirds.BMC neuroscience · 2014Article
- Cancer stem cell-specific scavenger receptor CD36 drives glioblastoma progression.Stem cells (Dayton, Ohio) · 2014Article
- The thrombospondin-1 receptor CD36 is an important mediator of ovarian angiogenesis and folliculogenesis.Reproductive biology and endocrinology : RB&E · 2014Article
- Pregnancy-associated genes contribute to antiluteolytic mechanisms in ovine corpus luteum.Physiological genomics · 2013Article
- Caffeine-induced endothelial cell death and the inhibition of angiogenesis.Anatomy & cell biology · 2013Article
- Endothelial expression of TNF receptor-1 generates a proapoptotic signal inhibited by integrin α6β1 in glioblastoma.Cancer research · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Thrombospondin-1 (TSP-1) treatment of dermal microvascular endothelial cells (MvEC) has been shown to upregulate Fas ligand (FasL) and to induce apoptosis by a mechanism that requires caspase-8 activity. We have examined the potential anti-angiogenic effects of TSP-1 on primary human brain MvEC. The addition of TSP-1 to primary human brain MvEC cultured as monolayers on type 1 collagen, induced cell death and apoptosis (evidenced by caspase-3 cleavage) in a dose- (5-30 nM) and time-dependent (maximal at 17 h) manner. TSP-1 treatment for 17 h induced caspase-3 cleavage that required caspase-8 activity and the tumor necrosis factor receptor 1 (TNF-R1). We did not find a requirement for Fas, or the tumor necrosis-related apoptosis-inducing ligand receptors (TRAIL-R) 1 and 2. We confirmed the findings using caspase inhibitors, blocking antibodies and small interfering RNA (siRNA). Further analysis indicated that the TSP-1 induction of caspase-3 cleavage of primary human brain MvEC adherent to collagen required the synthesis of new message and protein, and that TSP-1 induced the expression of TNFalpha mRNA and protein. Consistent with these findings, when the primary human brain MvEC were propagated on collagen gels mAb anti-TNF-R1 reversed the inhibitory effect, in part, of TSP-1 on tube formation and branching. These data identify a novel mechanism whereby TSP-1 can inhibit angiogenesis-through induction of apoptosis in a process mediated by TNF-R1.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.