ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2008
Isolation and characterization of a low phytic acid rice mutant reveals a mutation in the rice orthologue of maize MIK.
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 66 citations in OpenAlex.
- Identification of a novel mutation in theFrontiers in plant science · 2024Article
- GC-MS profiling and antibacterial activity ofBulletin of the National Research Centre · 2022Article
- A Systemic View of Carbohydrate Metabolism in Rice to Facilitate Productivity.Plants (Basel, Switzerland) · 2021Article
- AnPlants (Basel, Switzerland) · 2020Article
- Expression regulation of myo-inositol 3-phosphate synthase 1 (INO1) in determination of phytic acid accumulation in rice grain.Scientific reports · 2019Article
- Mutation ofPlants (Basel, Switzerland) · 2019Article
- Novel allelic variant of Lpa1 gene associated with a significant reduction in seed phytic acid content in rice (Oryza sativa L.).PloS one · 2019Article
- Development and Molecular Characterization of Low Phytate Basmati Rice Through Induced Mutagenesis, Hybridization, Backcross, and Marker Assisted Breeding.Frontiers in plant science · 2019Article
- Manipulating the Phytic Acid Content of Rice Grain Toward Improving Micronutrient Bioavailability.Rice (New York, N.Y.) · 2018Review
- Biofortification in Millets: A Sustainable Approach for Nutritional Security.Frontiers in plant science · 2017Review
- Tissue-specific expression, developmentally and spatially regulated alternative splicing, and protein subcellular localization of OsLpa rice.Journal of Zhejiang University. Science. B · 2016Article
- Seed Biofortification and Phytic Acid Reduction: A Conflict of Interest for the Plant?Plants (Basel, Switzerland) · 2015Review
- Seed-specific silencing of OsMRP5 reduces seed phytic acid and weight in rice.Transgenic research · 2014Article
- Characterization of OsMIK in a rice mutant with reduced phytate content reveals an insertion of a rearranged retrotransposon.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2013Article
- An uncharacterized member of the ribokinase family in Thermococcus kodakarensis exhibits myo-inositol kinase activity.The Journal of biological chemistry · 2013Article
- Development of low phytate rice by RNAi mediated seed-specific silencing of inositol 1,3,4,5,6-pentakisphosphate 2-kinase gene (IPK1).PloS one · 2013Article
- Identification and characterization of the soybean IPK1 ortholog of a low phytic acid mutant reveals an exon-excluding splice-site mutation.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2012Article
- Identification of genes necessary for wild-type levels of seed phytic acid in Arabidopsis thaliana using a reverse genetics approach.Molecular genetics and genomics : MGG · 2011Article
- A nonsense mutation in a putative sulphate transporter gene results in low phytic acid in barley.Functional & integrative genomics · 2011Article
- Iron fortification of rice seeds through activation of the nicotianamine synthase gene.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
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5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a forward genetics approach, we isolated two independent low phytic acid (lpa) rice mutants, N15-186 and N15-375. Both mutants are caused by single gene, recessive non-lethal mutations, which result in approximately 75% (N15-186) and 43% (N15-375) reductions in seed phytic acid (inositol hexakisphosphate). High-performance liquid chromatography and GC-MS analysis of seed extracts from N15-186 indicated that, in addition to phytic acid, inositol monophosphate was significantly reduced whereas inorganic phosphorus and myo-inositol were greatly increased when compared with wild-type. The changes observed in N15-186 resemble those previously described for the maize lpa3 mutant. Analysis of N15-375 revealed changes similar to those observed in previously characterized rice lpa1 mutants (i.e. significant reduction in phytic acid and corresponding increase in inorganic phosphorus with little or no change in inositol phosphate intermediates or myo-inositol). Further genetic analysis of the N15-186 mutant indicated that the mutation, designated lpa N15-186, was located in a region on chromosome 3 between the microsatellite markers RM15875 and RM15907. The rice orthologue of maize lpa3, which encodes a myo-inositol kinase, is in this interval. Sequence analysis of the N15-186 allele of this orthologue (Os03g52760) revealed a single base pair change (C/G to T/A) in the first exon of the gene, which results in a nonsense mutation. Our results indicate that lpa N15-186 is a mutant allele of the rice myo-inositol kinase (OsMIK) gene. Identification and characterization of lpa mutants, such as N15-186, will facilitate studies on the regulation of phytic acid biosynthesis and accumulation and help address questions concerning the contribution of the inositol lipid-dependent and independent biosynthetic pathways to the production of seed phytic acid.
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