ArticleProceedings of the National Academy of Sciences of the United States of America2008
Efficient reproduction of cynomolgus monkey using pronuclear embryo transfer technique.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 32 citations in OpenAlex.
- Article
- Non-human primate: the new frontier model of female reproductive engineering.Frontiers in bioengineering and biotechnology · 2025Review
- Study of tree shrew biology and models: A booming and prosperous field for biomedical research.Zoological research · 2024Review
- Comparative analysis of superovulated versus uterine-embryo synchronized recipients for embryo transfer in cynomolgus monkeys (Frontiers in veterinary science · 2024Article
- Rhesus monkeys exhibiting spontaneous ritualistic behaviors resembling obsessive-compulsive disorder.National science review · 2023Article
- Biology and postnatal development of organ systems of cynomolgus monkeys (Macaca fascicularis).Journal of medical primatology · 2023Review
- A controlled ovarian stimulation procedure suitable for cynomolgus macaques.Experimental animals · 2022Article
- The Spiny Mouse-A Menstruating Rodent to Build a Bridge From Bench to Bedside.Frontiers in reproductive health · 2021Review
- Genome editing of CCR5 by CRISPR-Cas9 in Mauritian cynomolgus macaque embryos.Scientific reports · 2020Article
- Generation of cynomolgus monkey fetuses with intracytoplasmic sperm injection based on the MII-stage oocytes acquired by personalized superovulation protocol.Journal of veterinary science · 2020Article
- One-step generation of complete gene knockout mice and monkeys by CRISPR/Cas9-mediated gene editing with multiple sgRNAs.Cell research · 2017Article
- Homology-mediated end joining-based targeted integration using CRISPR/Cas9.Cell research · 2017Article
- Efficient production of cynomolgus monkeys with a toolbox of enhanced assisted reproductive technologies.Scientific reports · 2016Article
- Autism-like behaviours and germline transmission in transgenic monkeys overexpressing MeCP2.Nature · 2016Article
- Generation of macaques with sperm derived from juvenile monkey testicular xenografts.Cell research · 2016Article
- Generation of a monkey with MECP2 mutations by TALEN-based gene targeting.Neuroscience bulletin · 2014Article
- Generation of haploid embryonic stem cells from Macaca fascicularis monkey parthenotes.Cell research · 2013Article
- Progress and prospects for genetic modification of nonhuman primate models in biomedical research.ILAR journal · 2013Review
- Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the technical bottlenecks in producing nonhuman primate models is that current assisted reproductive techniques, such as in vitro culture and frozen conservation of multicell-stage embryos, often result in poor embryo quality and subsequently lead to low birth rates. We investigated whether pronuclear embryo transfer can be used as an effective means for improving pregnancy and live birth rates of nonhuman primates. We collected 174 metaphase II oocytes by laparoscopy from 22 superovulated mature females and then fertilized these eggs using either in vitro fertilization or intracytoplasmic sperm injection, resulting in a 33.3% and a 50% fertilization rate, respectively. These 66 fertilized pronuclear-stage embryos were then tubally transferred to 30 recipients and led to 7 births and 1 abortion. Importantly, we observed that the highest live birth rate of approximately 64% was obtained when the transfer of pronuclear embryos was performed in the presence of new corpus luteum in the ovary of recipients between 24 h and 36 h after estradiol peak. Therefore, our experiments demonstrate that by matching the critical time window in the recipient's reproductive cycle for achieving optimal embryo-uterine synchrony, pronuclear embryo transfer technology can significantly improve the pregnancy rate and live birth of healthy baby monkeys. This efficient method should be valuable to the systematic efforts in construction of various transgenic primate disease models.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.