ArticleDevelopment (Cambridge, England)2008
Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst.
Article in Development (Cambridge, England), 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 279 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
279 citing papers in PubMed, 1 synthesis or guideline pooled it, 561 citations in OpenAlex.
- Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates.Frontiers in veterinary science · 2021Pooled it
- TEAD4 Regulates apical domain homeostasis and cell-positioning to maintain the trophectoderm lineage during mouse blastocyst development.Open biology · 2026Article
- Molecular Regulation of Post-Hatching Embryo Development: Implications for Early Pregnancy Losses in Livestock Species.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Harnessing the murine inner cell mass mechanical environment enhances derivation of in vitro nascent primitive endoderm precursor cells.Development (Cambridge, England) · 2026Article
- Oscillatory co-expression of HES1 and HES5 enables a hybrid state in a cross-repressive transcription factor regulatory motif.Development (Cambridge, England) · 2026Article
- Epitranscriptomic regulation of mRNA stability in pivotal transcription factors modulates the second cell fate decision.Nature communications · 2026Article
- Optimality as a framework for understanding developmental robustness.Trends in cell biology · 2026Review
- Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.bioRxiv : the preprint server for biology · 2026Article
- Generation of Blastoids from Human Naïve Pluripotent Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Wnt/β-catenin signalling modulates the timing of cell fate decision making in the early mouse embryo.PloS one · 2026Article
- Porcine cells restrict human cell proliferation via cellular competition in a human-porcine mesenchymal stem cells co-culture model.Frontiers in cell and developmental biology · 2026Article
- Boundary-guided cell alignment drives mouse epiblast maturation.Nature physics · 2026Article
- Molecular and epistatic interactions between pioneer transcription factors shape nucleosome dynamics and cell differentiation.Nature communications · 2025Article
- Monitoring rapid degradation of NANOG reveals UTP15 maintains pluripotency by regulating nascent transcripts.Nature communications · 2025Article
- Self-organization underlies developmental robustness in plants.Cells & development · 2025Review
- Primitive means first, not worst: Critical roles for primitive endoderm in embryos and embryo models.Developmental biology · 2025Review
- Review
- Stage-sensitive potential of isolated rabbit ICM to differentiate into extraembryonic lineages†.Biology of reproduction · 2025Article
- Broad H3K4me3 domains support oocyte genome silencing and maturation but are dispensable for repression in early embryos.Development (Cambridge, England) · 2025Article
- NANOG is repurposed after implantation to repress Sox2 and begin pluripotency extinction.The EMBO journal · 2025Article
219 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
The first two lineages to differentiate from a pluripotent cell population during mammalian development are the extraembryonic trophectoderm (TE) and the primitive endoderm (PrE). Whereas the mechanisms of TE specification have been extensively studied, segregation of PrE and the pluripotent epiblast (EPI) has received comparatively little attention. A current model of PrE specification suggests PrE precursors exhibit an apparently random distribution within the inner cell mass of the early blastocyst and then segregate to their final position lining the cavity by the late blastocyst. We have identified platelet-derived growth factor receptor alpha (Pdgfralpha) as an early-expressed protein that is also a marker of the later PrE lineage. By combining live imaging of embryos expressing a histone H2B-GFP fusion protein reporter under the control of Pdgfra regulatory elements with the analysis of lineage-specific markers, we investigated the events leading to PrE and EPI lineage segregation in the mouse, and correlated our findings using an embryo staging system based on total cell number. Before blastocyst formation, lineage-specific factors are expressed in an overlapping manner. Subsequently, a gradual progression towards a mutually exclusive expression of PrE- and EPI-specific markers occurs. Finally, cell sorting is achieved by a variety of cell behaviours and by selective apoptosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.