ArticleInfection and immunity2008
Escherichia coli O157:H7 survives within human macrophages: global gene expression profile and involvement of the Shiga toxins.
Article in Infection and immunity, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
32 citing papers in PubMed, 65 citations in OpenAlex.
- Global expression profile of enterohemorrhagic Escherichia coli O157:H7 in phagosome of murine macrophages.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026Article
- Carbon monoxide-producing probioticiScience · 2026Article
- High-risk clonal groups of avian pathogenicVirulence · 2025Article
- Pathogenomes of Shiga Toxin Positive and NegativeMicroorganisms · 2024Article
- Atypical diarrhoeagenicEpidemiology and infection · 2023Article
- Characterization of the Tellurite-Resistance Properties and Identification of the Core Function Genes for Tellurite Resistance inMicroorganisms · 2022Article
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- Pathogenomes of Atypical Non-shigatoxigenicFrontiers in microbiology · 2020Article
- Clever Cooperation: Interactions Between EspF and Host Proteins.Frontiers in microbiology · 2018Review
- Type VI secretion system contributes to Enterohemorrhagic Escherichia coli virulence by secreting catalase against host reactive oxygen species (ROS).PLoS pathogens · 2017Article
- NrdR Transcription Regulation: Global Proteome Analysis and Its Role in Escherichia coli Viability and Virulence.PloS one · 2016Article
- Small heat-shock proteins, IbpAB, protect non-pathogenic Escherichia coli from killing by macrophage-derived reactive oxygen species.PloS one · 2015Article
- The gluconeogenesis pathway is involved in maintenance of enterohaemorrhagic Escherichia coli O157:H7 in bovine intestinal content.PloS one · 2014Article
- Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 2013Article
- Proteomic View of Interactions of Shiga Toxin-Producing Escherichia coli with the Intestinal Environment in Gnotobiotic Piglets.PloS one · 2013Article
- Article
- Adaptive mutations and replacements of virulence traits in the Escherichia coli O104:H4 outbreak population.PloS one · 2013Article
- Article
- Human intestinal epithelial cell-derived molecule(s) increase enterohemorrhagic Escherichia coli virulence.FEMS immunology and medical microbiology · 2012Article
- Subtilase cytotoxin enhances Escherichia coli survival in macrophages by suppression of nitric oxide production through the inhibition of NF-κB activation.Infection and immunity · 2012Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Escherichia coli O157:H7 is an important food-borne pathogen that specifically binds to the follicle-associated epithelium in the intestine, which rapidly brings this bacterial pathogen in contact with underlying human macrophages. Very little information is available about the interaction between E. coli O157:H7 and human macrophages. We evaluated the uptake and survival of strain EDL933 during infection of human macrophages. Surprisingly, EDL933 survived and multiplied in human macrophages at 24 h postinfection. The global gene expression profile of this pathogen during macrophage infection was determined. Inside human macrophages, upregulation of E. coli O157:H7 genes carried on O islands (such as pagC, the genes for both of the Shiga toxins, and the two iron transport system operons fit and chu) was observed. Genes involved in acid resistance and in the SOS response were upregulated. However, genes of the locus of enterocyte effacement or genes involved in peroxide resistance were not differentially expressed. Many genes with putative or unknown functions were upregulated inside human macrophages and may be newly discovered virulence factors. As the Shiga toxin genes were upregulated in macrophages, survival and cytotoxicity assays were performed with isogenic Shiga toxin mutants. The initial uptake of Shiga toxins mutants was higher than that of the wild type; however, the survival rates were significantly lower at 24 h postinfection. Thus, Shiga toxins are implicated in the interaction between E. coli O157:H7 and human macrophages. Understanding the molecular mechanisms used by E. coli to survive within macrophages may help in the identification of targets for new therapeutic agents.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.